课题基金 / 基金详情

Adaptation mechanism of membrane lipids and its genetic control

Adaptation mechanism of membrane lipids and its genetic control
膜脂适应机制及其遗传调控
批准号:
61480465
负责人:
NOZAWA Yoshinori
金额:
$3.97万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1986
资助国家:
日本
项目状态:
已结题
起止时间:
1986 至 1988

项目摘要

项目成果

NOZAWA Yoshinori的其他基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
Temperature shift of Tetrahymena cells from 39 C to 15 C induced alterations in fatty acid composition of membrane phospholipids, a decrease in satureated palmitic acid and increases in unsaturated fatty acids such as -linoleinic and linoleic acids, causing its plasma membrane more fluid as inferred with ESR and freeze-fracture electron microscopy. Although low-temperature acclimation did not affect the viability of the cells, aggregation of monosome to form polysome was observed. Whether the increase in polysome content was derived from newly transcribed mRNA or not is to be clarified. Two dimensio- l gel electrophoretic analyses of microsomal proteins from low-temperature-shifted cells showed time-dependent increases in tubulin and some proteins which have lower apparent molecular masses than fatty acyl CoA-desaturase. Using two coding regions of rat liver stearyl-CoA desaturase cDNA, Southern blotting of Tetrahymena DNA was performed. Under low stringency of 35% formamide and 5xSSC, at 28 C for 24hr, EcoRI-digested DNA hybridized with one of the desaturase cDNA fragment. Furthermore, EcoRV- and BglII-digested DNA showed hybridizing bands of 2.6kb and 2.7kb, respectively, indicating a possibility of cloning Tetrahymena enzymes using rat-derived DNA as a probe.
期刊论文(60)
专著(0)
科研奖励(0)
会议论文
Nozawa,Y.: "Advances in Membrane Fluidity;Regulation of Membrane Fluidity in Unicellular Organisms" Aloia,R.C.(ed.)A,Alan R.Liss,Inc., 20 (1987)
Nozawa,Y.:“膜流动性的进展;单细胞生物膜流动性的调节”Aloia,R.C.(ed.)A,Alan R.Liss,Inc., 20 (1987)
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Umeki,L.: Biol,Chem.357. 61-65 (1986)
Umeki,L.:生物,化学.357。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Nozawa,Y.: "Regulation of membrane fluidity in unicellular organisms in "Physiological regulation of membrane fluidity"" Alan R.Liss,Inc, 18 (1988)
Nozawa,Y.:“膜流动性的生理调节”中单细胞生物膜流动性的调节”Alan R.Liss,Inc, 18 (1988)
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
31
    REGULATORY MECHANISM BY PHOSPHOLIPASE D IN OXIDANT-STRESS INDUCED SURVIVAL SIGNALING
    CROSS-TALK OF MEMBRANE LIPID SIGNALING IN CELL DEATH AND SURVIVAL
    MECHNISM OF APOPTOSIS INDUCED BY MEMBRANE LIPID SYGNALING
    • 批准号:
      12470042
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $7.94万
    • 财政年份:
      2000
    • 负责人:
      NOZAWA Yoshinori
    • 依托单位:
    Functional analysis of the new signal transduction enzyme PLD by the molecular genetic technique