Molecular mechanisms for regulation and physiological role of phospholipase D
磷脂酶D调节的分子机制和生理作用
基本信息
- 批准号:09480162
- 负责人:
- 金额:$ 9.6万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
It has been known that phospholipase D (PLD) has been implicated in a variety of signal transduction pathways, including secretion, vesicle trafficking, mitosis and meiosis. However, the physiological role of PLD in cell functions has not yet been elucidated. In this research project, we have investigated the role of PLD in various cell functions, such as cell growth, differentiation and apoptosis. We have cloned three PLD genes (rPLD1a, 1b, 2) from rat P012 cell and their chromosome mapping were performed by fluorescent in situ hybridization (FISH). The levels of mRNA of PLD isozymes (rPLD1a, 1b, 2) were examined in the course of the apoptotic process induced by C2-ceramide in rat basophilic leukemia RBL cells and glioma C6 cells. The mRNA levels of PLD1a, and PLD1b were greatly decreased whereas the level of PLD2 was in contrast increased. Two forms of PLD (ARF- and oleate-dependent PLDs) have been found to be present in nuclei isolated from rat hepatocytes The ADP-ribosylation factor (ARF)-dependent PLD, but not oleate-dependent PLD activity was increased in the S-phase of the regenerating rat liver after partial hepatectomy, suggesting that the nuclear ARF-dependent PLD may be associated with cell proliferation. The oleate-dependent PLD activity was abundantly present in rat P012 cells, which may be regulated by mitogen-activated protein kinase stimulated by [1202. The oleate-dependent PLD activity also was increased during Actinomycine D-induced Jurkat T cell apoptosis. These results suggest that distinct PLD isozymes may be involved in different cell functions.
已知磷脂酶D(PLD)参与多种信号转导途径,包括分泌、囊泡运输、有丝分裂和减数分裂。然而,PLD在细胞功能中的生理作用尚未阐明。在本研究项目中,我们研究了PLD在多种细胞功能中的作用,如细胞生长、分化和凋亡。我们从大鼠P012细胞中克隆了3个PLD基因(rPLD1a、1b、2),并用荧光原位杂交(FISH)对其进行了染色体定位。检测C2-神经酰胺诱导大鼠嗜碱性粒细胞白血病RBL细胞和胶质瘤C6细胞凋亡过程中PLD同工酶(rPLD1a、1b、2)的mRNA水平。PLD1a、PLD1b基因表达水平显著降低,而PLD2基因表达水平明显升高。在大鼠肝细胞核中发现了两种形式的PLD(ARF依赖的PLD和油酸依赖的PLD)。在肝部分切除后的S期再生肝中,依赖于ADP核糖化因子的PLD而不是油酸依赖的PLD的活性增加,提示核内依赖ARF的PLD可能与细胞增殖有关。油酸依赖的PLD活性在大鼠P012细胞中大量存在,这可能受[1202]刺激的丝裂原活化蛋白激酶的调节。在放线菌素D诱导Jurkat T细胞凋亡的过程中,油酸依赖的PLD活性也增加。这些结果表明,不同的PLD同工酶可能参与不同的细胞功能。
项目成果
期刊论文数量(63)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakashima,S: "Handbook of Experimental Pharmacology,Vol.126," Spring-Verlag,Heidelberg, 16 (1997)
Nakashima,S:“实验药理学手册,第 126 卷”,Spring-Verlag,海德堡,16 (1997)
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- 通讯作者:
Oguchi,K.: "Enhancement of phospholipase D activity during differentiation in HL60 cells:involvement of RhoA translocation to membranes" J.Biol.Chem.272. 1990-1996 (1997)
Oguchi,K.:“HL60 细胞分化过程中磷脂酶 D 活性的增强:RhoA 易位至细胞膜的参与”J.Biol.Chem.272。
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- 影响因子:0
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Hayakawa,K.: "Decreased phospholipase Dactivities in ceramide-induced apoptosis of RBL-2H3 mast cells." Biomed.Res.19. 159-170 (1998)
Hayakawa,K.:“神经酰胺诱导的 RBL-2H3 肥大细胞凋亡中磷脂酶 D 活性降低。”
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- 影响因子:0
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- 通讯作者:
Yoshimura,Y.: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat glial cellsishimura" J.Neurochem.,. 69. 713-720 (1997)
Yoshimura,Y.:“神经酰胺诱导的大鼠神经胶质细胞凋亡过程中磷脂酶 D 的活性和 mRNA 水平的变化”J.Neurochem.,。
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NOZAWA Yoshinori其他文献
NOZAWA Yoshinori的其他文献
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{{ truncateString('NOZAWA Yoshinori', 18)}}的其他基金
REGULATORY MECHANISM BY PHOSPHOLIPASE D IN OXIDANT-STRESS INDUCED SURVIVAL SIGNALING
氧化应激诱导的生存信号传导中磷脂酶 D 的调节机制
- 批准号:
16390098 - 财政年份:2004
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
CROSS-TALK OF MEMBRANE LIPID SIGNALING IN CELL DEATH AND SURVIVAL
细胞死亡和存活中膜脂信号传导的交叉对话
- 批准号:
14370064 - 财政年份:2002
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
MECHNISM OF APOPTOSIS INDUCED BY MEMBRANE LIPID SYGNALING
膜脂信号诱导细胞凋亡的机制
- 批准号:
12470042 - 财政年份:2000
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analysis of the new signal transduction enzyme PLD by the molecular genetic technique
分子遗传学技术分析新型信号转导酶PLD的功能
- 批准号:
10212204 - 财政年份:1998
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas (B)
Molecular mechanisms for membrane lipid signaling in apoptosis
细胞凋亡中膜脂信号传导的分子机制
- 批准号:
07457036 - 财政年份:1995
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on Functions of bioactive phospholipids
生物活性磷脂的功能研究
- 批准号:
06304050 - 财政年份:1994
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Action mechanisms and roles of small GTP-binding proteins
小 GTP 结合蛋白的作用机制和作用
- 批准号:
05271103 - 财政年份:1993
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Studies on the conformation and functions of ras-related low Mr GTP-binding proteins.
ras相关低Mr GTP结合蛋白的构象和功能研究。
- 批准号:
03304052 - 财政年份:1991
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
Interaction of multiple phospholipase C and GTP-binding proteins in platelet signal transduction
多种磷脂酶 C 和 GTP 结合蛋白在血小板信号转导中的相互作用
- 批准号:
02454544 - 财政年份:1990
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Adaptation mechanism of membrane lipids and its genetic control
膜脂适应机制及其遗传调控
- 批准号:
61480465 - 财政年份:1986
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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