Action mechanisms and roles of small GTP-binding proteins

小 GTP 结合蛋白的作用机制和作用

• 批准号: 05271103
• 负责人: NOZAWA Yoshinori
• 金额: $ 111.87万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05271103/
• 关键词: Small GTP-binding protein; Vesicle transport; ADP-ribosylation; Phosphorylation; Signal transduction; 酵母

RhoA and PKC activate synergistically the membrane-bound phospholipase D (PLD) in HL60. The Toxin B and C3 toxin treatments show involvement of CDC42 in PLD activation in RBL cells. PLD cDNAs were cloned from rat (Nozawa). (2) Rhabphilin-3A binds to synaptic vesicles via anchoring protein. A newly identified homolog Doc regulates exocytosis. A target protein of Rho1 is PKC in budding yeast (Takai). (3) Target proteins of Rho are PKN and p160^<PRCK>, rhophilin, rhotekin and citron. PKN and p160^<PRCK> are activated by GTP/Rho (Narumiya). (4) Expression of RhoA and RhoGDI decreases with age in rat prostate. The 20 kDa protein (H3 histone) is ADP-ribosylated (Ishibashi). (5) A factore inbolved in Raf1 activation is identified to be 14-3-3. A MAP kinase kinase (TAK1) acts in TGF-beta signaling (Matumoto). (6) Sar1p and E112K participate in vesicle transport between endoplasm reticulum and Golgi body in yeast. SAR1 from tobacco rescues SAR1 defective mutant of yeast (Nakano). (7) A arginine-specific ADP-ribosyl transferase cloned from chiken PMN ADP-ribosylates Arg^<28> in actin (Shimoyama). (8) A novel inhibitor (CKB-1055) inhibits S6 kinase in insulin signaling and exerts insulin-like action in glycogen metabolism (Hidaka.) (9) PLA_2 inhibitor blocks fusion of Golgi bodies and vesicles leading to inhibition of secretion. Brefeldin blocks vesicle formation (Ikehara). (10) Gene expression of thymidine transports greatly repressed in DnaA defective mutant of E.coli. (Sekimizu).

RhoA和PKC协同激活HL60的膜结合磷脂酶D （PLD）。毒素B和C3毒素处理表明CDC42参与了RBL细胞PLD的激活。从大鼠（Nozawa）克隆PLD cdna。(2) Rhabphilin-3A通过锚定蛋白与突触囊泡结合。一种新发现的同系物Doc调节胞吐。Rho1的靶蛋白是出芽酵母中的PKC。(3) Rho的靶蛋白为PKN和p160^<PRCK>、rhotekin、rhotekin和citron。PKN和p160^<PRCK>被GTP/Rho （Narumiya）激活。(4)大鼠前列腺中RhoA和RhoGDI的表达随年龄的增长而降低。20 kDa蛋白（H3组蛋白）被adp核糖化（Ishibashi）。(5)鉴定出一个参与Raf1激活的因子为14-3-3。MAP激酶激酶（TAK1）在tgf - β信号传导中起作用。(6)酵母中Sar1p和E112K参与了内质网与高尔基体之间的囊泡运输。来自烟草的SAR1拯救了酵母SAR1缺陷突变体（中野）。(7)鸡PMN adp -核糖基转移酶Arg^<28> (Shimoyama)。(8)一种新的抑制剂（CKB-1055）抑制胰岛素信号通路中的S6激酶，并在糖原代谢中发挥胰岛素样作用（Hidaka.）(9) PLA_2抑制剂阻断高尔基体与囊泡的融合，抑制高尔基体的分泌。布雷菲尔丁阻断囊泡形成（Ikehara）。（10）大肠杆菌dna缺陷突变体胸苷转运基因表达受到抑制。(Sekimizu)。

项目成果

Kurokawa T.: "Reduction of mono(ADP-ribosyl)ation of histones in rat testis by gonadotropin-testosterone system" Biochem. Biophys. Res. Commun.215. 808-815 (1995)

Kurokawa T.：“促性腺激素-睾酮系统减少大鼠睾丸中组蛋白的单（ADP-核糖基）化”Biochem。

Sato,E.: "Eraly changes in glucose metabolism in the cerebrum of senescence accelerated mouse." Brain Res.637. 133-138 (1994)

Sato,E.：“小鼠大脑中葡萄糖代谢的早期变化加速了小鼠的衰老。”

Takano,Y.: "Role of cytoplasmic tail phosphorylation sites of platelet-activating factor receptor in agonist-induced desensitization." J.Biol.Chem.269. 22453-22458 (1994)

Takano,Y.：“血小板激活因子受体细胞质尾部磷酸化位点在激动剂诱导的脱敏中的作用。”

Okimoto,T.: "Brefeldin A protects ricin-induced cytotoxicity in human cancer KB cell line,but not in its resistant counterpart with altered Golgi structures." Cell Struct.Funct.18. 241-251 (1993)

Okimoto, T.：“布雷菲德菌素 A 可以保护人类癌症 KB 细胞系中蓖麻毒素诱导的细胞毒性，但不能保护高尔基体结构改变的耐药细胞系。”

Watanabe,T.: "Reduction of mono(ADP-ribosyl)action of histones in rat testis by gonadotropin-testosterone system" Science. 271. 645-648 (1995)

Watanabe，T.：“促性腺激素-睾酮系统减少大鼠睾丸中组蛋白的单（ADP-核糖基）作用”科学。