Studies on Transmission of Hemorrhagic Fever with Renal Syndrome (HFRS) Virus in Rodents

肾综合征出血热（HFRS）病毒在啮齿动物中传播的研究

• 批准号: 62480087
• 负责人: HASHIMOTO Nobuo
• 金额: $ 3.84万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480087/
• 关键词: Hemorrhagic fever with renal syndrome (HFRS); HFRS virus; Hantavirus; Rodent; Transmission of HFRS virus; Epizootiology of HFRS; Ecology of HFRS virus; Viral Zoonoses; げっ歯類媒介性人畜共通伝染病; ドブネズミ; ウィルス性ズノーシス; げつ菌類媒介性人畜共通伝染病; HFRSウイルス; げつ歯類; HFRSウイ

The mode of HFRS virus transmission among rodents was examined by comparing the results of epidemiological surveys of urban rats in endemic areas and experimental infection of laboratory rats. The results are as follows.1) The urban rats in the endemic focus have shown the age-dependent acquisition of infection. HFRS virus was mostly isolated from lung tissue in adult rats showing high antibody titers.2) A total four sero-positive small field mice, two Clethrionomys rufocanus bedfordiae, one Apodemus argenteus hokkaidi and one Apodemus speciosus ainu, were detected around the endemic focus indicating an evidence of transmission of HFRS virus from the infected urban rats to indigenous field mice.3) Two strains of HFRS virus from Rattus, SR-11 and KI-262, showed identical antigenicity but deferred in virulence against newborn rats. SR-11 strain isolated from a laboratory rat developed clinical signs and died at about 18 days after inoculation. KI-262 strain from an urban rat in an endemi … More c focus, did not show any clinical signs. Necrosis of neurons in the brain tissue occurred in the rats infected with SR-11, while it was mild in the animals infected with KI-262.4) When rats-were inoculated with SR-11, sero-conversion rate was almost similar in newborn and adult rats regardless of inoculation routes.5) IgG antibody was transferred from immune dams to their offspring prenatally as well as postnatally. IgG antibody was detected in the fetus sera. In newborn rats, maternal IgG antibody reached a peak titer at two weeks after birth, then declined and disappeared at about 8 weeks of age. The protective effect of maternal antibody remained in 10 week-old rats.6) Antibody dependent enhancement (ADE) of the infection of HFRS virus was studied using macrophage-like cell lines. ADE of the infection occurred with monoclonal antibodies (MAbs) to both Gl and G2 envelope glycoproteins, but not with MAbs to nucleocapsid protein. Antigenic determinants related to hemagglutination and virus neutralization were found to cause ADE of the infection Less

通过对疫区城市鼠的流行病学调查结果与实验鼠感染情况的比较，探讨HFRS病毒在啮齿动物中的传播方式。研究结果如下：1)疫源地城市鼠感染呈年龄依赖性获得。2)在疫源地周围共检出4只血清阳性的小野小鼠，其中2只为红纹夜蛾，1只为黑线姬鼠，1只为大姬鼠，表明HFRS病毒可从感染的城市大鼠传播给本地田鼠。3)家鼠的两株HFRS病毒SR-11和Ki-262具有相同的抗原性，但对新生大鼠的毒力较弱。从实验室大鼠分离的SR-11菌株出现临床症状，并在接种后约18天死亡。Endemi…中一只城市鼠的Ki-262菌株病灶多为c，未见任何临床体征。感染SR-11的大鼠脑组织出现神经元坏死，而感染Ki-262.4的大鼠脑内神经元坏死较轻)接种SR-11后，无论接种途径，新生大鼠和成年大鼠的血清转阴率几乎相同。5)免疫母鼠在出生前和出生后将免疫母鼠的抗体传递给后代。在胎儿血清中检测到免疫球蛋白抗体。在新生大鼠中，母血免疫球蛋白抗体在出生后2周达到高峰，然后下降并在8周左右消失。6)用巨噬细胞样细胞系研究了抗体依赖增强(ADE)对HFRS病毒感染的保护作用。抗G1和G2包膜糖蛋白的单抗均可发生感染，而抗核衣壳蛋白的单抗则无此反应。与血凝和病毒中和相关的抗原决定因素被发现导致感染的ADE较少。

项目成果

Hashimoto, N.: "Epidemiological surveys of hantavirus infections among urban rats in an endemic focus in Japan" 23rd Working Conference on Viral Diseases, Japan-U.S.Cooperative Medical Science Program, 23, 18(1989).

Hashimoto, N.：“日本流行病点城市老鼠中汉坦病毒感染的流行病学调查”第 23 届病毒病工作会议，日美合作医学科学计划，23, 18(1989)。

Hashimoto,N;Zhang,X.K.: Working Conference on Viral Diseases,U.S.-Japan Cooperative Medical Science Program,Monterey. 21. 25 (1987)

Hashimoto,N;Zhang,X.K.：病毒性疾病工作会议，美日合作医学科学计划，蒙特雷。

Zhang,X-K.,Takashima,I.and Hashimoto,N.: "Characteristics of passive immunity against hatavirus infection in rats" Arch.Virol.105. 235-246 (1989)

张X-K.，高岛，I.和桥本，N.：“大鼠针对哈他病毒感染的被动免疫特征”Arch.Virol.105。

Maeda, T., Arikawa, J., Takashima, I.and Hashimoto, N.: "Enzyme linked immunosorbent assay for detection of antibody against hemorrhagic fever with renal syndrome virus in sera from experimentally infected rats" Jpn.J.Vet.Res.35 215-219 (1987).

Maeda, T.、Arikawa, J.、Takashima, I. 和 Hashimoto, N.：“酶联免疫吸附测定法检测实验感染大鼠血清中抗肾综合征病毒出血热的抗体”Jpn.J.Vet.Res

Arikawa, J.and Hashimoto, N.: "Characterizations of hantaan virus envelope glycoprotein antigenic determinants related to virus neutralization and pathogenesis in suckling mice" 1st International Conference of HFRS, Seoul, 1, 3(1989).

Arikawa, J. 和 Hashimoto, N.：“与乳鼠病毒中和和发病机制相关的汉坦病毒包膜糖蛋白抗原决定簇的特征”第一届 HFRS 国际会议，首尔，1, 3(1989)。