Mechanism and Persistency of HFRS Virus Infection in Laboratory Animals.

实验动物中 HFRS 病毒感染的机制和持续性。

• 批准号: 01580043
• 负责人: DOHMAE Kayoko
• 金额: $ 1.28万
• 依托单位: Research Institute for Microbial Diseases, Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01580043/
• 关键词: Hemorrhagic Fever with Renal Syndrome (HFRS); Korean Hemorrhagic Fever (KHF); Mouse; Rat; HFRS inactivated Vaccine B-1 Strain; 腎症候性出血熱HFRS; 不活性化ワクチンBー1

The protective effect of antibodies could be transferred from immune dams to newborn. The effect of maternal antibody raised by immunization with HFRS vaccine on the protection of newborn rats and mice from infection by HFRS virus was examined. Antibody to HFRS virus was transferred from immune dams to their offspring prenatally as well as postnatally. In the sera of the newborn, IgG and IgA titer of maternal antibody were detected and reached a park around 1 week after birth, then declined and disappeared at about 14 weeks of age. After intraperitoneal challenge by B-1 or KHF virus. Death and infection of 1-day-old rats or mice from immune dams were prevented by the presence of maternal antibody. The protective effect of maternal antibody is limited only to saving the life of newborns and is not to prevent viral infection when titer of maternal antibody is low. Further persistent infection of HFRS virus was demonstrated by the existence of virus in the brain of old rats infected more than 3 years ago.

抗体的保护作用可从免疫母体转移到新生儿。研究了肾综合征出血热疫苗免疫所产生的母源抗体对新生大鼠和小鼠感染肾综合征出血热病毒的保护作用。肾综合征出血热病毒抗体在出生前和出生后从免疫母鸡传递给后代。新生儿血清中母源抗体的Ig G和Ig A滴度在出生后1周左右达到一个公园，14周龄左右下降并消失。B-1或KHF病毒腹腔攻击后。母源抗体的存在可防止1日龄大鼠或小鼠从免疫母体中死亡和感染。母源抗体的保护作用仅限于挽救新生儿生命，在母源抗体效价较低时不能预防病毒感染。3年前感染的老年大鼠脑内存在病毒，进一步证实了HFRS病毒的持续感染。

项目成果

堂前 嘉代子: "腎症候性出血熱ウイルス、及び関連ウイルス、の新生仔マウス、新生仔ラットに対する病原性の比較検討" 和歌山医学. 40. (1989)

Kayoko Domae：“肾症状出血热病毒和相关病毒对新生小鼠和大鼠的致病性的比较研究”和歌山医学40。（1989）

H.Asada,K.Balachandra,H.Tamura,K.Kondo,and K.Yamanishi: "Crossーreactive Immunity among Different Serotypes of Virus Causing Haemorrhagic Fever with Renal Syndrome." J.gen.Virol.70. 819-825 (1989)

H. Asada、K. Balachandra、H. Tamura、K. Kondo 和 K. Yamanishi：“导致肾综合征出血热的不同血清型病毒之间的交叉反应免疫”，J.gen.Virol.70。 (1989)

M.Tamura, H.asada, K.Kondo, O.Tanisita, and K.Yamanishi: "Pathogenesis of Hantaan Virus in Mice." J.gen.Virol.70. 2897-2906 (1989)

M.Tamura、H.asada、K.Kondo、O.Tanisita 和 K.Yamanishi：“汉滩病毒在小鼠中的发病机制”。

K. Dohmae: "Comparative Studies on the Virulence of Hemorrhagic Fever with Renal Syndrome (HFRS) on Newborn Mice and Rats." Wakayamaigaku. 40. 1-13 (1989)

K. Dohmae：“肾综合征出血热 (HFRS) 对新生小鼠和大鼠毒力的比较研究。”

M. Tamura et al: "Pathogenesis of Hantaan Virus in Mice." J. gen. Virol.70. 2897-2906 (1899)

M. Tamura 等人：“汉坦病毒在小鼠中的发病机制”。