The Mechanism of Abortion caused by Arboviruses in Domestic Animals and the Ecology of the Viruses

家畜虫媒病毒引起流产的机制及病毒生态

• 批准号: 63304027
• 负责人: HASHIMOTO Nobuo
• 金额: $ 7.23万
• 依托单位: Faculty of Veterinary Medicine, Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63304027/
• 关键词: Arbovirus; Abortion; Viral abortion; Abortion in domestic animals; Congenital abnormality; Mechanism of congenital abnormality caused by arbovirus; Viral congenital abnormality; Ecology of arbovirus; ウィルス性異常産; ウィルス性奇形の発現機序; 節足動物媒介性ウィルス; 3.アルボウイ

The mechanism of abortion in domestic animals caused byarboviruses, Japanese encephalitis virus (JEV), Getah virus (GTV), Akabane virus (AKV), Chuzan virus (CZV) and bovine viral diarrhoea-mucosal disease virus (BVD-MDV) was studied by virological, pathological and epizzotiological examinations.1) Outbreaks of abortion and stillbirth caused by JEV were observed in swine population in Hokkaido. JE antibody positive farms were found at the same site in negative farms in 3 years continuously. This result indicates that the JEV was maintained in a distinct endemic focus.2) Vertical transmission of JE virus in Aedes japonicas was demonstrated with a minimum rate of 0.7%.3) Dot-immunobinding assay has been evaluated as a useful method for the detection of anti-GTV antibody in horses.4) Pathological studies were performed on bovine Akabane disease occurred in Iwate. Arthrogriposis was recognized in 14 out of 70 cattle involved in the occurrence.5) AKV was isolated from the biting midge, Culicooides oxystoma, collected in a cowshed in Kagoshima.6) Chuzan virus classified as a new member of the Playah subgroup of the genus Orbivirus was isolated from healthy calves and from Culiooides oxystoma. The virus caused epidemic of congenital abnormalities of calves characterized by the hydroencephally-cerebellar hydroplasia which occurred in Kyushu.7) When cattle persistently infected with non-cytopathic BVD-MDV were challenged to cytopathid strain, some of them developed mucosal disease and morbund. All control cattle showed transit fever but no diarrhea, and recovered from infection.8) Two newborn calves with congenital malformation by in utero infection with BVD-MDV also showed ataxia and blindness. At autopsy, cerebellar hypoplasia and reduction insize of the optic nerves were observed in both cases in addition to severe hydrocephalus in one case.

本文从病毒学、病理学和流行病学等方面研究了虫媒病毒、日本脑炎病毒（JEV）、盖塔病毒（GTV）、赤羽病毒（AKV）、中山病毒（CZV）和牛病毒性脑炎-粘膜病病毒（BVD-MDV）引起家畜流产的机理。连续3年在同一地点发现乙脑抗体阳性猪场。结果表明，乙脑病毒在日本伊蚊中的垂直传播率最低为0.7%;斑点免疫结合试验是一种检测马抗GTV抗体的有效方法;从鹿儿岛牛舍采集的尖口库蚊（Culicooides oxostoma）中分离到AKV;从健康小牛和尖口库蚊中分离到Chuzan病毒，该病毒被归类为环状病毒属Playah亚组的新成员。该病毒引起了以九州发生的脑-小脑积水为特征的小牛先天性畸形的流行。7）当持续感染非细胞病变性BVDV-MDV的牛用细胞病变性毒株攻毒时，其中一些牛发生了粘膜疾病和病态。所有对照牛均出现过境热，但无腹泻，并从感染中恢复。8）2头因子宫内感染BVDV-MDV而先天畸形的新生小牛也出现共济失调和失明。尸检时，除了一例严重脑积水外，在两例病例中均观察到小脑发育不全和视神经缩小。

项目成果

清宮幸男、伊藤博、及川団、小川富男、大池裕治、大島寛一、岡田幸助: "牛ウイルス性下痢、粘膜病ウイルスの胎内感染に起因する先天性異常を有する新生子牛2例の疫学および病理学的観察" 日本獣医師会雑誌. 41. 725-730 (1988)

Yukio Kiyomiya、Hiroshi Ito、Dan Oikawa、Tomio Okawa、Yuji Oike、Kanichi Oshima、Kosuke Okada：“两只新生犊牛的流行病学和疾病，患有牛病毒性腹泻和子宫内感染粘膜疾病病毒引起的先天性畸形。“身体观察”日本兽医协会杂志 41. 725-730 (1988)

Shimizu, M., Watanabe, H., Satou, K. and Murakami, S.: "Antigenic diversity of bovine viral diarrhea-mucosal disease (BVD-MD) viruses recently isolated from persistently infected cattle and mucosal disease, and serologic survey on bovine sera using antige

Shimizu, M.、Watanabe, H.、Satou, K. 和 Murakami, S.：“最近从持续感染的牛和粘膜疾病中分离出的牛病毒性腹泻-粘膜疾病 (BVD-MD) 病毒的抗原多样性以及血清学调查

Seimiya, Y., Ito, H., Oikawa, M., Obara, T., Oh-ike, Y., Ohshima, K.and Okada, K.: "Epizootiology and pathology of congenital malformations in two newborn calves caused by intrauterine infection with bovine viral diarrheamucosal disease virus" Jpn J. Vet.

Seimiya, Y.、Ito, H.、Oikawa, M.、Obara, T.、Oh-ike, Y.、Ohshima, K. 和 Okada, K.：“两只新生牛犊先天性畸形的流行病学和病理学

Shimizu, M.: "Transplacetal infection of bovine viral diarrhea-mucosal disease (BVD-MD) : on the persistent infection" Dosei Kyokai Kaiho. 21. 13-19 (1988 (in Japanese))

Shimizu, M.：“牛病毒性腹泻粘膜疾病（BVD-MD）的经胎盘感染：关于持续感染”Dosei Kyokai Kaiho。

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