Molecular genetics of subactute sclerosing panencephalitis (SSPE) virus

亚急性硬化性全脑炎（SSPE）病毒的分子遗传学

• 批准号: 62480157
• 负责人: YAMANOUCHI Kazuya
• 金额: $ 3.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480157/
• 关键词: SSPE virus; Measles virus; Molecular genetics; Neurovirulence; Persistent infection; Neurotoxin; Pathogenesis; Slow virus infection; ブンガロトキシン; HA蛋白; アセチルコリンレセプタ-; PーMmRNA; NPーL mRNA; 麻疹ウイルスts株

(1) Gene analysis of SSPE virus, Yamagata-1 strain, was conducted. The entire base sequences of M, NP, H, and F genes as well as part of the sequences of L and P genes were determined.(2) Di-cistronic mRNA of NP-L was most abundantly observed in cDNA library. It was classified into two types; the one consisting of 865 bp of NP and 1293 bp of L, and the other consisting of 187 bp of NP and 1192 bp of L. Such di-cistronic mRNA has not been reported thus far.(3) The amino acid sequence of the carboxy terminus corresponding to the end of the outer envelope projection of HA of SSPE virus was found to have a high degree of homology with the active domain of long neurotoxin which specifically binds to the nicotini acetylcholine.receptor. This domain was shown to cross-react with HA of SSPE virus and of measles virus antigenically. In addition, SSPE patients had significantly high titers of antibody to this domain. Thus, this epitope of HA is considered to play an important role in the transsynaptic spreading of the virus in the brain.

(1)对SSPE病毒Yamagata-1株进行了基因分析。测定了M、NP、H、F基因的全部碱基序列以及L、P基因的部分序列。(2)文库中发现的NP-L的双顺反子序列最为丰富。该基因分为两类，一类是NP的865bp和L的1293bp，另一类是NP的187bp和L的1192bp，这种双顺反子基因至今未见报道。(3)SSPE病毒HA外膜投影末端的羧基末端氨基酸序列与与烟碱乙酰胆碱受体特异结合的长神经毒素活性结构域高度同源。该结构域与SSPE病毒的HA和麻疹病毒的HA在抗原性上有交叉反应。此外，SSPE患者对该区域的抗体效价明显较高。因此，HA的这一表位被认为在病毒在大脑中的跨突触传播中发挥着重要作用。

项目成果

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Y. Morikawa et al.: "Characterization of canine distemper viruses adapted to human neural cells." Microbiol. Immunol., 32, 1211-1220, 1988.

Y. Morikawa 等人：“适应人类神经细胞的犬瘟热病毒的特征。”

Timothy C.Wong: "Generalized and Localized Biased Hypermutation Affecting the Matrix Gene of a Measles Virus Strain That Causes Subacute Sclerosing Panencephalitis" Journal of Virology. 63. 5464-5468 (1989)

Timothy C.Wong：“影响导致亚急性硬化性全脑炎的麻疹病毒株基质基因的全身性和局部性偏向超突变”病毒学杂志。

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