Study on proportional effect of life span exposure of air pollutant (NO_2) on respiratory tumor carcinogenesis.

终生暴露空气污染物(NO_2)对呼吸道肿瘤致癌作用的比例效应研究。

• 批准号: 62480181
• 负责人: MURAKAMI Masataka
• 金额: $ 2.56万
• 依托单位: National Institute for Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480181/
• 关键词: NITROGEN DIOXIDE; LIFE SPAN EXPOSURE; TUMOR PROMOTION EFFECTS; N-bis(2-hydroxypropyl) nitrosamine; ADENOMA; ADENOCARCINOMA; PALMANARY EPITHERIAL CELLS; HYPERPLASIA; 慢性暴露; プロモーション作用; N-bis(2+hydroxypropyl) nitrosoamine(DHPN); ポリアミン; オルニチンデカルボキシ

The effects of nitrogen dioxide (NO_2) on lung tumorigenesis by N-bis(2-hydroxypropyl) nitrosamine (DHPN) in male Wistar rats Were investigated. Rats were injected with o.5 g DHPN per kg body weight or saline intraperitoneally one time at 6 weeks of age, and then exposed to clean air, 0.04 ppm or 4 ppm NO for 17 months. Incidence of tumor in lungs of rats in DHPN + 4 ppm NO_2, group was 13%, and the tumors were adenoma and adenocarcinoma.Adenoma was seen in 4 of 40 rats (10%) and adenocarcinoma was seen in I of 40 rats (3%).The incidence of tumor in lungs of rats in DHPN + clean air and DHPN + 0.04 ppm NO_2 groups was only 3% (1/40), respectively. The tumors were adenoma. There was no significant difference in the incidence of tumors between DHPN + clean air group and DHPN + 4 ppm NO_2 group. No lung tumor was seen in DHPN + 0.4 ppm group and each NO_2-exposed group without DHPN treatment. Alveolar cell hyperplasia occurred at the highest incidence in.lungs of rats injected with DHPN. But, there was no any correlation between the incidence of hyperplasia and each concentration of NO_2 exposed. Bronchiole mucosal hyperplasia was seen in 17 of rats (43%) in the DHPN + 4 ppm NO_2 group, and in I of 40 rats (3%) in the each clean air, 0.04 ppm or 0.4 ppm NO_2 exposed-groups with DHPN treatment. The hyper- plasia in lungs of rats exposed to 4 ppm NO_2 without DHPN treatment was more slighter than that seen in the 4 ppm NO_2-exposed group with DHPN treatment.These results indicate that development of lung tumors in rats injected with subcarcino- genic dose of DHPN was-enhanced by exposure to 4 ppm NO_2. However, low levels of NO_2 effect on lung tumor development was not observed in Wistar rats.

研究了二氧化氮(NO2)对N-二(2-羟丙基)亚硝胺(DHPN)诱导的雄性Wistar大鼠肺肿瘤形成的影响。6周龄大鼠每公斤体重注射0.5g DHPN或生理盐水一次，然后暴露于清洁空气、0.04ppm或4ppm NO中，持续17个月。DHPN+4ppm NO2组大鼠肺部肿瘤发生率为13%，肿瘤为腺瘤和腺癌，腺瘤发生率为10%(4/40)，腺癌发生率为3%(I/40)，DHPN+清洁空气组和DHPN+0.04ppmNO2组大鼠肺部肿瘤发生率分别为3%(1/40)。肿瘤均为腺瘤。DHPN+洁净空气组与DHPN+4ppm NO2组肿瘤发生率差异无统计学意义。DHPN+0.4ppm组和未经DHPN处理的各NO2暴露组均未见肺肿瘤。DHPN组大鼠肺组织中肺泡细胞增生发生率最高。但不同浓度的NO2暴露与牙周组织增生的发生率无明显相关性。DHPN+4ppm NO_2组有17只大鼠(43%)出现细支气管壁粘膜增生，DHPN+0.04ppm或0.4ppm NO_2暴露组各有40只大鼠(3%)出现细支气管壁增生。未经DHPN处理的4ppm NO2染毒大鼠肺组织的增生程度明显轻于经DHPN处理的4ppmNO2染毒组。结果表明，注射致癌剂量DHPN的大鼠暴露于4ppmNO2可促进肺癌的发生，而低浓度的NO2对大鼠肺肿瘤的发生无明显影响。

项目成果

嵯峨井勝: Medio(Medical view Comp.). 5. 69-74 (1988)

Masaru Sagai：Medio（医学观点比较）。5. 69-74 (1988)。

H.P.Witschi、嵯峨井勝: 大気汚染学会誌. 24. 1-20 (1989)

H.P.Witschi，Masaru Sagai：《空气污染学会杂志》24. 1-20 (1989)。

嵯峨井勝,市瀬孝道,佐野友春,村上正孝,藤井敬二: "二酸化窒素の発癌促進作用に関する実験的研究" 大気汚染学会誌(in press. 26. (1990)

Masaru Sagai、Takamichi Ichise、Tomoharu Sano、Masataka Murakami、Keiji Fujii：“二氧化氮致癌作用的实验研究”日本空气污染学会杂志（出版中。26。（1990）

Hamapeter Witschi,嵯峨井勝: "オゾンおよび二酸化窒素と肺がんーー最近の知見と問題点ーー" 大気汚染学会誌. 24. 1-20 (1989)

Hamapeter Witschi、Masaru Sagai：“臭氧、二氧化氮和肺癌 - 最近的发现和问题”日本空气污染学会杂志 24. 1-20 (1989)。

嵯峨井勝,市瀬孝道,佐野友春,村上正孝,藤井敬二: "二酸化窒素の発癌促進作用に関する実験的研究" 大気汚染学会誌. 26. (1990)

Masaru Sagai，Takamichi Ichise，Tomoharu Sano，Masataka Murakami，Keiji Fujii：“二氧化氮致癌作用的实验研究”日本空气污染学会杂志26。（1990）。