The induction mechanisms for the paracellular transport upon stimulation of salivary secretion

刺激唾液分泌后细胞旁转运的诱导机制

• 批准号: 23590271
• 负责人: MURAKAMI Masataka
• 金额: $ 3.33万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590271/
• 关键词: 上皮機能; 傍細胞輸送; タイト結合; 血管灌流唾液腺; 水輸送; 細胞内信号; 蛍光物質分泌; 駆動力; 細胞内骨格

To clarify the mechanism to start the paracellular transport (more than 60% of the whole fluid secretion of the gland is estimated from paracellular route), the isolated vascularly perfused mandibular gland of rat was placed on the stage of the confocal laser-scanning microscope. We observed the passage of the fluorescent dye (~500 Da) as an indicator of paracellular transport. When the fluid secretion was induced by muscarinic or alpha1-adrenergic stimulation, the fluorescence of each intracellular canalliculi responded variously, but the average of canalliculus increased dose-dependently to the plateau within 30s. The present project led an conclusion that we can observe the entry of fluorescent dye from intercellular space to luminal space by pressure-dependent paracellular route by solvent-drag, and that the fluid secretion through pressure-independent route is still open for further studies. The process of opening continued for 30 s, which was similar to that of cell shrinkage.

为了阐明启动细胞旁转运的机制（估计超过60%的腺体的全部液体分泌来自细胞旁途径），将离体血管灌注的大鼠下颌腺置于共聚焦激光扫描显微镜的台上。我们观察到荧光染料（~500 Da）的通过，作为细胞旁转运的指标。当M或α 1-肾上腺素能刺激引起液体分泌时，细胞内各小管的荧光反应不同，但小管的平均值在30 s内呈剂量依赖性增加至平台。本项目的结论是，我们可以观察到荧光染料从细胞间隙进入腔空间的压力依赖性旁路途径的溶剂拖动，和液体分泌通过压力非依赖性途径仍然是开放的进一步研究。开放过程持续30 s，与细胞收缩过程相似.

项目成果

ラット顎下腺細胞間分泌細管の微細形態:急速凍結固定割断試料の電顕観察と高速共焦点レーザ顕微鏡観察

大鼠颌下腺细胞分泌小管的微形态：快速冷冻、固定和切片样品的电子显微镜和高速共聚焦激光显微镜

• 发表时间: 2013
• 作者: 村上政隆;魏飛;成田貴則;福島美和子;橋本貞充;澁川義幸;佐藤正樹
• 通讯作者: 佐藤正樹

The actin-specific reagent jasplakinolide induces apoptosis in primary rat parotid acinar cells.

• DOI: 10.1016/j.archoralbio.2011.10.014
• 发表时间: 2012-05
• 期刊: Archives of oral biology
• 影响因子: 3
• 作者: M. Matsuki-Fukushima;S. Hashimoto;M. Murakami;Y. Ogata;J. Fujita-Yoshigaki;Takanori Narita;H. Sugiya

Mn-citrate and Mn-HIDA: intermediate-affinity chelates for manganese-enhanced MRI.

• DOI: 10.1002/cmmi.1510
• 发表时间: 2013-03
• 期刊: Contrast media & molecular imaging
• 作者: Y. Seo;Keitaro Satoh;H. Morita;A. Takamata;Kazuto Watanabe;T. Ogino;T. Hasebe;M. Murakami

静水圧依存性水分泌に及ぼすムスカリン/βアドレナリン受容体刺激の影響

毒蕈碱/β-肾上腺素能受体刺激对静水压依赖性水分泌的影响

• 发表时间: 2011
• 作者: 成田貴則;祁兵;村上政隆;杉谷博士.;村上政隆;魏飛;瀬尾芳輝;魏飛,村上政隆;村上政隆,魏飛
• 通讯作者: 村上政隆,魏飛

Salivary fluid secretion induced by chinese herb, Danshen

中药丹参诱导唾液分泌

• 发表时间: 2011
• 作者: 成田貴則;祁兵;村上政隆;杉谷博士.;村上政隆;魏飛;瀬尾芳輝;魏飛,村上政隆;村上政隆,魏飛;Murakami M;Wei F,Murakami M
• 通讯作者: Wei F,Murakami M