The induction mechanisms for the paracellular transport upon stimulation of salivary secretion

为了阐明启动细胞旁转运的机制（估计超过60%的腺体的全部液体分泌来自细胞旁途径），将离体血管灌注的大鼠下颌腺置于共聚焦激光扫描显微镜的台上。我们观察到荧光染料（~500 Da）的通过，作为细胞旁转运的指标。当M或α 1-肾上腺素能刺激引起液体分泌时，细胞内各小管的荧光反应不同，但小管的平均值在30 s内呈剂量依赖性增加至平台。本项目的结论是，我们可以观察到荧光染料从细胞间隙进入腔空间的压力依赖性旁路途径的溶剂拖动，和液体分泌通过压力非依赖性途径仍然是开放的进一步研究。开放过程持续30 s，与细胞收缩过程相似.

To clarify the mechanism to start the paracellular transport (more than 60% of the whole fluid secretion of the gland is estimated from paracellular route), the isolated vascularly perfused mandibular gland of rat was placed on the stage of the confocal laser-scanning microscope. We observed the passage of the fluorescent dye (~500 Da) as an indicator of paracellular transport. When the fluid secretion was induced by muscarinic or alpha1-adrenergic stimulation, the fluorescence of each intracellular canalliculi responded variously, but the average of canalliculus increased dose-dependently to the plateau within 30s. The present project led an conclusion that we can observe the entry of fluorescent dye from intercellular space to luminal space by pressure-dependent paracellular route by solvent-drag, and that the fluid secretion through pressure-independent route is still open for further studies. The process of opening continued for 30 s, which was similar to that of cell shrinkage.