The induction mechanisms for the paracellular transport upon stimulation of salivary secretion

为了阐明启动细胞旁转运的机制(腺体全部分泌物的60%以上是通过细胞旁途径估计的)，将大鼠离体血管灌流的下颌腺置于激光共聚焦扫描显微镜的舞台上。我们观察到荧光染料(~500Da)的通过，作为细胞旁运输的指示剂。当刺激M胆碱或α1-肾上腺素能刺激时，各小管的荧光反应不同，但30s内小管的平均荧光强度随剂量的增加而增加。本项目的结论是，我们可以观察到荧光染料通过压力依赖的细胞旁途径通过溶剂-阻力进入细胞间隙，而通过压力非依赖途径的液体分泌仍有待进一步研究。30岁的S继续着这个开放的过程，这类似于细胞收缩的过程。

To clarify the mechanism to start the paracellular transport (more than 60% of the whole fluid secretion of the gland is estimated from paracellular route), the isolated vascularly perfused mandibular gland of rat was placed on the stage of the confocal laser-scanning microscope. We observed the passage of the fluorescent dye (~500 Da) as an indicator of paracellular transport. When the fluid secretion was induced by muscarinic or alpha1-adrenergic stimulation, the fluorescence of each intracellular canalliculi responded variously, but the average of canalliculus increased dose-dependently to the plateau within 30s. The present project led an conclusion that we can observe the entry of fluorescent dye from intercellular space to luminal space by pressure-dependent paracellular route by solvent-drag, and that the fluid secretion through pressure-independent route is still open for further studies. The process of opening continued for 30 s, which was similar to that of cell shrinkage.