Production of useful tumor markers for gyvecologic tumors.

生产妇科肿瘤有用的肿瘤标志物。

• 批准号: 62480346
• 负责人: INOUE Masaki
• 金额: $ 4.42万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480346/
• 关键词: Tumor marken; Monoclonal Antibody; Gynecologic Tumor; PRHA; Fucose transterase; Blood group antigen; Canbohydrate antigen; Immunohisto chemistry; Fucose Transferase; 腫瘍マーカー; モノクローナル抗体; 子宮内膜癌; 糖鎖異常

A murine monoclonal antibody ,MCA-97,was prepared against a human endometrial adenocarcinoma cell line. In cellular enzyme-linked immunospecific assay,the MCA-97 antibody reacted with all adenocarcinoma cell lines tested including four endometrial adenocarcinoma lines. The antigen defined by MCA-97 was estimated to be a non sialylated high-molecular-weight glycoprotein containing galactose and N-acetylglucosamine in its determinant. In immunoperoxidase staining of formalin-fixed, paraffin-embedded sections,MCA-97 reacted with most endometrial adenocarcinomas and ovarian endometrial-type adenocarcinomas, and also reacted with normal glandular epithelium of the female genital and gastrointestimal tracts. However, it was mostly unreactive with squarous cell carcinoma and ovarian serous adenocarcinoma tissues. By the reversed passive hemagglutination method, the antigen defined by MCA-97 was detected in the sera of one-third of patients with endometrioid adenocarcinomas, and in half of those with ovarian endometrial adenocarcinamas. The Antigen was not demonstrable in sera of most normal female volunteers. Thus, MCA-97 has potential clinical applications in diagnostic serology and pathology.

针对人子宫内膜腺癌细胞系制备了鼠单克隆抗体MCA-97。在细胞酶连接的免疫特异性测定中，MCA-97抗体与所有测试的腺癌细胞系反应，其中包括四个子宫内膜腺癌系。据估计，由MCA-97定义的抗原是其决定因素中含有半乳糖和N-乙酰葡萄糖胺的非溶解的高分子糖蛋白。在福尔马林固定的，石蜡包裹的部分的免疫过氧化物酶染色中，MCA-97与大多数子宫内膜腺癌和卵巢子宫内膜型腺癌反应，也与正常的女性生殖器和气体膜膜的腺上皮反应。然而，它主要与良好的细胞癌和卵巢浆液性腺癌组织无反应。通过反向的被动性血凝法，在三分之一的子宫内膜类腺癌患者的血清中检测到MCA-97定义的抗原，其中一半患有卵巢子宫内膜腺癌的患者中有一半。在大多数正常女性志愿者的血清中，抗原无法证明。因此，MCA-97在诊断血清学和病理学中具有潜在的临床应用。

项目成果

Inoue M,Ogawa H Nakanishi K,et al: "Clinical value of sialyl Tn antiger in patients with gynecologic tumors." Obstet Gynecol(in press). 74. (1990)

Inoue M,Okawa H Nakanishi K,et al：“唾液酸 Tn 抗原在妇科肿瘤患者中的临床价值。”

Kimura T,Inoue M,Miyake A,et al: "The use of serum TA-4 in monitoring patients with malignant trausformation of ovarian mature cystic teratoma" Cancer. 64. 480-483 (1989)

Kimura T、Inoue M、Miyake A 等人：“血清 TA-4 在监测卵巢成熟囊性畸胎瘤恶性创伤患者中的应用”癌症。

Inoue M,Nakayama M,Tanizawa O: "Altered expression of Lewis blood group and related antigens in fetal,normal adult and malignant tissues of the uterine endometrium" Virchows Archier A Pathological Anatomy and histopathology. 416. 221-228 (1990)

Inoue M，Nakayama M，Tanizawa O：“Lewis 血型和相关抗原在胎儿、正常成人和子宫内膜恶性组织中表达的改变” Virchows Archier A 病理解剖学和组织病理学。

Inoue M, Nakayama M, Tanizawa O: "Altered expression of Lewis blood group and related antigens in tetal, normal adult and malignant tissues of the uterine endometrium." Vir chows Archieu A Pathological Anatomy and Histopathology.416:221-228, 1990.

Inoue M、Nakayama M、Tanizawa O：“Lewis 血型和相关抗原在子宫内膜组织、正常成人和恶性组织中的表达发生改变。”

Inoue,M: Gynecologic Oncology.

Inoue，M：妇科肿瘤学。