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Immunological studies of local antitumor effects by X ray irradiation

X射线照射局部抗肿瘤作用的免疫学研究

基本信息

批准号：
62480405
负责人：
SAKUDA Masayoshi
金额：
$ 2.94万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480405/
关键词：
Local irradiation Transplantable tumor 癌免疫

项目摘要

In order to investigate the effect of radiotherapy on the immunity in the tumor-bearing mice, the status of general immunity and local immune reaction were studied after irradiation of transplanted Meth-A tumor cells.After local irradiation against the tumors transplanted onto the legs, the growth of the non-irradiated tumors transplanted into opposite side legs was suppressed. It was suggested that these anti-tumor effects after local tumor irradiation resulted from the anti-tumor immunity by mouse spleen lymphocytes, especially natural killer(NK)and t cells. At the same time, NK activity of spleen lymphocyte was also increased. The histological findings showed that lymphocyte infiltration into the tumor tissue was recognized at the earlier stage and more markedly in the non-irradiated opposite side leg tumor tissue of mice, of which leg tumors in another side were irradiated,than in the tumor tissue of non-irradiated mice. The immunohistological analysis revealed that the subpopulations of these infiltrated lymphocytes were composed of mainly NK and T cells.Still more, the growth of tumors at the pre-irradiated tissue were studied. Tumor growth at the pre-irradiated legs was suppressed in any cases of high-dose or low-dose irradiation.It is suggested that, in the low-dose irradiation, the anttumor immunity mainly acted on the suppression of the tumor growth, and in the high-dose irradiation, suppression of the tumor growth was mainly induced by both the antitumor immunity and the radiation damage to blood vessels which was recognized in the histological analysis by the image analyzer.

为探讨放射治疗对荷瘤小鼠免疫功能的影响，本实验观察了移植性Meth-A肿瘤细胞经放射治疗后的全身免疫和局部免疫反应状况，结果表明：移植瘤局部照射后，未照射的移植瘤生长受到抑制。提示肿瘤局部照射后的抗肿瘤作用是由小鼠脾淋巴细胞，尤其是自然杀伤（NK）细胞和t细胞产生的抗肿瘤免疫作用所致。同时，脾淋巴细胞NK活性也明显增强。组织学结果表明，淋巴细胞浸润到肿瘤组织中是在早期阶段被识别的，并且在未照射的另一侧腿部肿瘤的小鼠的对侧腿部肿瘤组织中比在未照射的小鼠的肿瘤组织中更明显，其中另一侧腿部肿瘤被照射。免疫组织化学分析表明，这些浸润的淋巴细胞主要由NK细胞和T细胞组成。高剂量和低剂量照射均能抑制照射前小腿肿瘤的生长，提示低剂量照射时，抗肿瘤免疫主要起抑制肿瘤生长的作用，而高剂量照射时，肿瘤生长的抑制主要是由抗肿瘤免疫和血管的辐射损伤引起的，这在组织学分析中通过图像分析仪。