Mechanism on the rise and fall of NK activity and CTL in tumor bearing mice in comparison with cryosurgery.

与冷冻手术相比，荷瘤小鼠 NK 活性和 CTL 升降的机制。

• 批准号: 60480434
• 负责人: SAKUDA Masayoshi
• 金额: $ 2.62万
• 依托单位: Osaka University , Faculty of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480434/
• 关键词: Tumor immunity; NK activity; CTL; CTL

In this studies, the relationship between NK activity and cytotoxic T lymphocytes (CTL) were examined in splenic lymphocytes of tumor bearing mice.Furthermore, mechanisms on the rise and fall of NK activity and CTL were investigated after inoculation of cryo-destructed tumor cells.On the bases of these results, the usefulness of cryosurgery was discussed from a standpoint of tumor immunotherapy.[ Results ] NKcells belong, at least in part, to the T cell lineage, and the NK cells might be converted to CTL, in the tumor bearing mice. This possible pathway is considered to be due to control with sialic acid of cell surface.Cryo-destructed tumor cells enhanced NK activity, and could not induced CTL. On the contrary, tumor cells treated with MMC induced low NK activity,and could induce CTL. It was suggested that these rise and fall of NK activity and CTL related to interferon, prostaglandin E , suppressor T cells or interleukin 2.Thus cryosurgery augmented the non-specific immunity such as NK activity,but could not induce tumor-specific immunity.

本研究检测了荷瘤小鼠脾淋巴细胞中NK活性与细胞毒性T淋巴细胞(CTL)的关系，并探讨了冻融肿瘤细胞接种后NK活性和CTL的变化机制。在此基础上，从肿瘤免疫治疗的角度探讨了冷冻治疗的可行性。[结果]在荷瘤小鼠中，NK细胞至少部分属于T细胞系，NK细胞可能转化为CTL。这一可能途径可能与细胞表面唾液酸调控有关。冷冻破坏的肿瘤细胞增强了NK活性，不能诱导CTL。相反，经MMC处理的肿瘤细胞具有较低的NK活性，并可诱导CTL。提示NK活性和CTL的升高和下降与干扰素、前列腺素E、抑制性T细胞或白介素2有关。因此，冷冻手术增强了NK活性等非特异性免疫，但不能诱导肿瘤特异性免疫。

项目成果

中沢光博,森山知是,大野一夫,滝田正亮,額田純一郎,作田正義: Cryo surgery研究会誌. 10,11. 16-19 (1986)

Mitsuhiro Nakazawa、Chize Moriyama、Kazuo Ohno、Masaaki Takita、Junichiro Nukada、Masayoshi Sakuta：冷冻手术研究组杂志 10,11 (1986)。

T.Yoneda;M.Urade;M.Sakuda;T.Miyazaki: J.Clin.Invest.77. 1613-1621 (1986)

T.Yoneda；M.Urade；M.Sakuda；T.Miyazaki：J.Clin.Invest.77。

額田純一郎,岩本忍,森山知是,作田正義: 日本口腔科学会雑誌. 35. 158-163 (1986)

Junichiro Nukata、Shinobu Iwamoto、Chise Moriyama、Masayoshi Sakuta：日本口腔医学会杂志 35. 158-163 (1986)。

T.Matsumura,T.Sugawara, S.Yui, S.Kobayashi, Y.Ishida, M.Nakamura and H.Fuchihata: "Effect of irradiation on lung metastasis of VX2 carcinoma in maxillary sinus of rabbit." Oral Radiology. 1. 117-123 (1986)

T.Matsumura、T.Sukawara、S.Yui、S.Kobayashi、Y.Ishida、M.Nakamura 和 H.Fuchihata：“照射对兔上颌窦 VX2 癌肺转移的影响”。

J.Nukata, S. Iwamoto, T.Moriyama and M.Sakuda: "Influences of OK-432 on distribution and traffic of mice splenic NK cells" J Jap stomatol Soc. 35. 158-163 (1986)

J.Nukata、S. Iwamoto、T.Moriyama 和 M.Sakuda：“OK-432 对小鼠脾脏 NK 细胞分布和运输的影响”J Jap stomatol Soc。