Antitumor activity of Porphyromonas gingivalis lipid A

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We investigated the antitumor activity and immunomodulatory effects of P.gingivalis lipid A.Periphera blood mononuclear cells obtained from healthy donors were incubated with Porphyromonas gingivalis lipid A(5Omcg/ml) in vitro. The supernatants were harvested and measured the activities of cytokines such as IL-1gamma, IL-1 receptor antagonist (IL-1ra), IL-6, IL-8, GM-CSF.TNF-alpha and IFN-gamma by ELISA assay. P.gingivalis lipid A induced considerably weaker IL-1gamma and TNF-alpha production than did E.coil LPS and its lipid A and salmonella-type lipid A.ft gingivalis lipid A induced higher IL-1ra production than did F.coil LPS and its lipid A.P.gingivalis LPS and its lipid A induced high levels of IL-6, IL-8 and GM-CSF.IFN-gamma production was more strongly induced by Rgingivalis LPS than by the other test specimens.Cytostasis by splenic macrophages stimulated with ft gingivails LPS and its lipid A were enhanced significantly in a dose-dependent manner. In the cytolytic assay, ft gingivalis lipid A exhibited tumoricidal activities, but weaker than its parent LPS.NK activities of P.gingivalis LPS and its lipid A were enhanced and almost equivalent to those obtained with E coil LPS and its lipid A.P.gingivails lipid A were failed to inhibit regional lymph node metastasis of MM48 mammary carcinoma. P.gingivalis LPS most strongly inhibited the lymph nodes metastases.These results suggested that ft gingivails lipid A had different immunological activities from those of enterobacterial lipid As, and induced enough level of antitumor immunity. P.gingivalis lipid A has a possibility of clinical use as a low-toxic immunomodulator, but it is necessary to investigate the effective administration methods in clinical model.