Light-Microscopic Analysis of Secretory Activity of Neurosecretory Cells

神经分泌细胞分泌活性的光显微镜分析

基本信息

  • 批准号:
    63480024
  • 负责人:
  • 金额:
    $ 4.48万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1988
  • 资助国家:
    日本
  • 起止时间:
    1988 至 1990
  • 项目状态:
    已结题

项目摘要

In the vertebrates, the hypothalamo-neurohypophysial neurosecretory system is involved in regulation of various physiological phenomena. However, it is not yet clear how synthesis and release of neurohypophysial hormones are controlled by external and internal environmental changes. Thus, in this research project, I have attempted to study this questions by use of two dimensional light microscopic photometric techniques, Synthetic activity of neurohypophysial hormones in individual neurosecretory cells has been determined by semiquantitative immunohistochemical and oligonucleotide-mRNA in situ hybridization (ISH) techniques. The ISH method used in the present project was established and developed in our laboratory. Furthermore, the nucleotide sequences of cDNAs encoding precursors of salmonid neurohypophysial hormones were analyzed in chum salmon and masu salmon so as to examine the above questions from comparative bases.Concerning effects of environmental osmotic changes on expression … More s of neurohypophysial hormone precursor genes, we have been able to obtain following results :1) After drinking 2% NaCl solution in rats, the level of provasopressin mRNA was significantly elevated in the supraoptic nucleus, but the change in the paraventricular nucleus was not significant. While water deprivation increased the provasopressin mRNA level in both the supraoptic and paraventricular nuclei.2) Transfer of seawater-adapted rainbow trout to fresh water elevated the level of provasotocin mRNA, whereas the inverse lowered the mRNA level.3) These results indicate that expression of genes encoding vasopressin-like peptides is elevated by a hyperosmotic stimulus in the rat, whereas it is stimulated by a hypoosmotic stimulus in the salmonid fish.Application of voltage-sensitive fluorescent dyes has been tested to record discharges of individual neurosecretory cells, since secretory activity of neurohypophysial hormones is well correlated to neuronal electric activity. I have not been able to complete this part, because of a delay in preparation of computer programs used in this technique. Less
在脊椎动物中,下丘脑-神经垂体神经分泌系统参与各种生理现象的调节。然而,神经垂体激素的合成和释放如何受到外部和内部环境变化的控制尚不清楚。因此,在本研究项目中,我试图利用二维光学显微镜光度技术对这一问题进行研究,采用半定量免疫组织化学和寡核苷酸-mRNA原位杂交(ISH)技术检测了单个神经分泌细胞中神经垂体激素的合成活性。本项目中使用的ISH方法是在我们实验室建立和发展的。此外,为了从比较的基础上检验上述问题,对鲑鱼和马苏鲑鱼中编码鲑鱼神经垂体激素前体的…的核苷酸序列进行了分析。了解环境渗透变化对表达的影响通过对神经垂体激素前体基因的更多研究,我们已经能够得到以下结果:1)大鼠饮用2%氯化钠溶液后,视上核加压素基因的表达水平显著升高,而室旁核的变化不明显。在缺水的情况下,视上核和室旁核的加压素基因表达水平增加。2)海水适应的虹鱼转移到淡水中可提高加压素基因的表达水平,反之亦然。3)这些结果表明,编码加压素样肽的基因在大鼠中受到高渗刺激的上调,而在鲑鱼中则受到低渗刺激的刺激。应用电压敏感荧光染料来记录单个神经分泌细胞的放电,因为神经垂体激素的分泌活动与神经元的电活动密切相关。我无法完成这一部分,因为这项技术中使用的计算机程序的准备工作出现了延误。较少

项目成果

期刊论文数量(33)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Urano, A.,: "In situ hybridization techniques in the study of endocrine secretions." Progress in Clinical and Biological Research. 342. 309-314 (1990)
Urano, A.,:“原位杂交技术在内分泌分泌物研究中的应用。”
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
A.Urano: "In situ hybridization techniques in the study of endocrine secretion" Progress in Comparative Endocrinology、1989. (1989)
A.Urano:“内分泌研究中的原位杂交技术”比较内分泌学进展,1989。(1989)
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    0
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Hyodo, S.,: "Moleculer- and immuno-histochemical study on expressions of vasopressin and oxytocin genes following water deprivation." Zoological Science. 6 :. 335-343 (1989)
Hyodo, S.,:“缺水后加压素和催产素基因表达的分子和免疫组织化学研究。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hyodo,S.: "Development of an in situ hybridization method for neurohypophysial hormone mRNAs using synthetic oligonucleotide probes." Zooiogical Science. 5. 397-406 (1988)
Hyodo,S.:“使用合成寡核苷酸探针开发神经垂体激素 mRNA 的原位杂交方法。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hyodo,S.: "Molecularー and immunoーhistochemical study on expressions of vasopressin and oxytocin genes following water deprivation." Zoological Science. 6. 335-343 (1989)
Hyodo, S.:“缺水后加压素和催产素基因表达的分子和免疫组织化学研究。动物学科学”6. 335-343 (1989)。
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    0
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URANO Akihisa其他文献

URANO Akihisa的其他文献

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{{ truncateString('URANO Akihisa', 18)}}的其他基金

Molecular Neuroanatomical Bases of Control of Salmonid Spawning Migration
控制鲑鱼产卵洄游的分子神经解剖学基础
  • 批准号:
    13440245
  • 财政年份:
    2001
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Basis for Neurohormonal Control of Salmonid Spawning Migration
鲑鱼产卵洄游神经激素控制的分子基础
  • 批准号:
    10102001
  • 财政年份:
    1998
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
Environmental factors regulating fish neurohormone genes
环境因素调节鱼类神经激素基因
  • 批准号:
    03454027
  • 财政年份:
    1991
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Multidisciplinary Research on Reproductive Biology in Japanese Toads
日本蟾蜍生殖生物学的多学科研究
  • 批准号:
    59340049
  • 财政年份:
    1984
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)

相似海外基金

The possibleprincipal function of neurohypophysial hormones
神经垂体激素可能的主要功能
  • 批准号:
    22570065
  • 财政年份:
    2010
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Evolution of Neurohypophysial Hormones and Their Functions
神经垂体激素的进化及其功能
  • 批准号:
    8315767
  • 财政年份:
    1984
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Continuing Grant
Role of Neurohypophysial Hormones in Oviposition
神经垂体激素在产卵中的作用
  • 批准号:
    65B2807
  • 财政年份:
    1965
  • 资助金额:
    $ 4.48万
  • 项目类别:
Role of Neurohypophysial Hormones on Oviposition and Water Metabolism in Chickens
神经垂体激素对鸡产卵和水代谢的作用
  • 批准号:
    6321180
  • 财政年份:
    1963
  • 资助金额:
    $ 4.48万
  • 项目类别:
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