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Environmental factors regulating fish neurohormone genes

环境因素调节鱼类神经激素基因

基本信息

批准号：
03454027
负责人：
URANO Akihisa
金额：
$ 4.22万
依托单位：
Hokkaido University (1993)The University of Tokyo (1991-1992)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454027/
关键词：
Salmonid Fish Hypothalamus Neurosecretion Vasotocin Isotocin GnRH Gene Expression Environmental factor

项目摘要

Expression of Neurohypophysial HOrmone Genes in Salmonids :Teleostean neurohypophysial hormones are considered to be vasotocin (VT) and isosotocin (IT). Since salmonids are tetraploid, they may usually have at least a pair of genes for precursors of proteinaceous hormones, including VT and IT precursor genes. Hence antisense oligodeoxynucleotides which can recognize both of 2 types of mRNAs were synthesized as probes for an in situ hybridization study on effects of increase in the level of pro-VT mRNA was found in preotic neurosecretory neurons, suggesting a possible role of VT in fresh water adaptarion.The pattern of expression of 2 pro-VT and 2 pro-IT genes were examined by use of Northern blot analysis in rainbow trout, masu salmon, sockeye salmon and chum salmon. The hybridization signal for pro-IT-I was stronger than that for pro-IT-II in all species examined, whereas the pattern of VT gene expression was different among species. Such information is quite important in a future study to analyze regulatory mechanisms of hormonal gene expression in salmonids.Expression of Adenohypophysial Hormone Genes :Changes in the levels of gonadotropin precursor genes were examined in rainbow trout following gonadal maturation. Expression of genes from growth harmone and prolactin was also examined in pituitaries of rainbow trout in primary culture.

鲑科鱼类神经垂体激素基因的表达：硬骨鱼类的神经垂体激素被认为是催产素（VT）和异催产素（IT）。由于鲑鱼是四倍体，它们通常至少有一对蛋白质激素前体基因，包括VT和IT前体基因。因此，我们合成了能同时识别这两种mRNA的反义寡核苷酸作为探针，通过原位杂交研究了前耳神经分泌神经元中pro-VT mRNA水平的增加，提示VT可能在淡水适应中起作用。红鲑和鲑鱼。在所有物种中，pro-IT-I的杂交信号均强于pro-IT-II，而VT基因的表达模式在物种间存在差异。这样的信息是相当重要的，在未来的研究中分析的激素基因表达的调控机制，在salmonids.Expression腺垂体激素基因：在虹鳟鱼性腺成熟后的促性腺激素前体基因的水平的变化进行了检查。生长激素和催乳素基因的表达也在原代培养的虹鳟鱼垂体中进行了检测。