Behavior of Langerhans Cells in the Hemopoietic Tissue, Skin, and Lymph Nodes.

朗格汉斯细胞在皮肤相关免疫系统中发挥抗原提呈功能。研究了朗格汉斯细胞在小鼠皮肤、口腔粘膜、前胃和淋巴结中的行为。用ATPase组织化学方法和免疫组织化学方法对表皮和粘膜上皮片进行Ia抗原染色，以显示朗格汉斯细胞。用电子显微镜观察Birbeck颗粒的出现情况。本文研究了郎格罕细胞和Thy-L阳性细胞在前胃上皮和表皮中的分布关系。观察了禽类皮肤表皮中ATPase阳性朗格汉斯样细胞的出现情况。这些研究的结果总结如下：1)未成熟的朗格汉斯细胞在胎儿时期进入表皮。这些细胞是ATPase阳性，但Ia抗原阴性。2)出生后1周内，这些细胞在细胞膜上表达Ia抗原，并在胞浆内形成Birbeck颗粒。它们的数量在出生后一周内增加到成人皮肤的频率水平。3)然后它们开始通过淋巴管到达区域淋巴结，并到达皮质旁区域，在那里它们与淋巴细胞或间质(ID)细胞进行密切接触。4)3周龄前粘膜内有少量朗格汉斯细胞。从那以后，他们的数量开始增加。5)在前胃粘膜中，朗格汉斯细胞在上皮细胞之间延伸出长的树突，每个细胞占据很大的空间。6)郎格汉斯细胞与Thy-L阳性细胞之间有密切的分布关系。7)在禽类表皮中发现了ATPase阳性的朗格汉斯样细胞。

Langerhans cells perform an antigen presenting function in the skin-associated immune system. The behavior of the Langerhans cells was studied in the mouse skin, oral mucous membrane, forestomach, and lymph nodes. To demonstrate the Langerhans cells, epidermal and mucosal epithelial sheets were stained by ATPase histochemistry and immunohistochemical methods for Ia antigens. The occurrence of Birbeck granuls was examined by electron microscopy. The distributional relationship between the Langerhans cells and Thy-l-positive cells was investigated in the epidermis and the epithelium of forestomach. The occurrence of ATPase-positive, Langerhans-like cells was investigated in the epidermis of avian skin. The results of these studies were summarized as follows : 1) Immature Langerhans cells enter the epidermis during the fetal period. These cells are ATPase-positive, but Ia antigen negative. 2) Within 1 week after birth, these cells express Ia antigen on their cell membrane and form Birbeck granules in their cytoplasm. They increase in number to the level of the frequency of adult skin by 1 week after birth. 3) They then begin to travel to the regional lymph node via lymphatic vessells, and reach the paracortical area, where they make close contacts with lymphocytes or Inter=Digitating (ID) cells. 4) In the mucous membrane Langerhans cells occur in small numbers before 3 weeks of age. They begin to increase in number thereafter. 5) In the mucous membrane of forestomach, Langerhans cells extend long dendritic processes between the epithelial cells, thus each cell occupies a large space. 6) There is an intimate distributional relationship between Langerhans cells and Thy-l-positive cells. 7)In the avian epidermis ATPase-positive Langerhans-like cells were discovered in the present study.