Behavior of Langerhans Cells in the Hemopoietic Tissue, Skin, and Lymph Nodes.

造血组织、皮肤和淋巴结中朗格汉斯细胞的行为。

• 批准号: 63480095
• 负责人: HOSHINO Takeshi
• 金额: $ 3.65万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480095/
• 关键词: Langerhans cells; Cell behavior; Mouse; Skin; Lymph nodes; Oral mucous membrane; Forestomach; Bird skin; 皮膚関連免疫系; Thy-1陽性細胞; Ia抗原; 個体発生

Langerhans cells perform an antigen presenting function in the skin-associated immune system. The behavior of the Langerhans cells was studied in the mouse skin, oral mucous membrane, forestomach, and lymph nodes. To demonstrate the Langerhans cells, epidermal and mucosal epithelial sheets were stained by ATPase histochemistry and immunohistochemical methods for Ia antigens. The occurrence of Birbeck granuls was examined by electron microscopy. The distributional relationship between the Langerhans cells and Thy-l-positive cells was investigated in the epidermis and the epithelium of forestomach. The occurrence of ATPase-positive, Langerhans-like cells was investigated in the epidermis of avian skin. The results of these studies were summarized as follows : 1) Immature Langerhans cells enter the epidermis during the fetal period. These cells are ATPase-positive, but Ia antigen negative. 2) Within 1 week after birth, these cells express Ia antigen on their cell membrane and form Birbeck granules in their cytoplasm. They increase in number to the level of the frequency of adult skin by 1 week after birth. 3) They then begin to travel to the regional lymph node via lymphatic vessells, and reach the paracortical area, where they make close contacts with lymphocytes or Inter=Digitating (ID) cells. 4) In the mucous membrane Langerhans cells occur in small numbers before 3 weeks of age. They begin to increase in number thereafter. 5) In the mucous membrane of forestomach, Langerhans cells extend long dendritic processes between the epithelial cells, thus each cell occupies a large space. 6) There is an intimate distributional relationship between Langerhans cells and Thy-l-positive cells. 7)In the avian epidermis ATPase-positive Langerhans-like cells were discovered in the present study.

朗格汉斯细胞在皮肤相关免疫系统中执行抗原呈递功能。在小鼠皮肤、口腔粘膜、前胃和淋巴结中研究朗格汉斯细胞的行为。为了显示朗格汉斯细胞，表皮和粘膜上皮片通过ATP酶组织化学和Ia抗原的免疫组织化学方法染色。Birbeck颗粒的发生通过电子显微镜检查。在前胃表皮和上皮中，观察了郎格罕细胞和Thy-1阳性细胞的分布关系。研究了禽皮肤表皮中ATP酶阳性朗格汉斯样细胞的发生。结果表明：1）胎儿时期，未成熟的朗格汉斯细胞进入表皮。这些细胞是ATP酶阳性，但Ia抗原阴性。2)出生后1周内，这些细胞在其细胞膜上表达Ia抗原，并在其细胞质中形成Birbeck颗粒。它们在出生后1周增加到成人皮肤频率的水平。3)然后，它们开始通过淋巴管到达局部淋巴结，并到达副皮质区，在那里它们与淋巴细胞或间指（ID）细胞密切接触。4)在粘膜中，郎格罕氏细胞在3周龄之前出现在少量中。此后，它们的数量开始增加。5)在前胃粘膜中，朗格汉斯细胞在上皮细胞之间延伸长的树突状突起，因此每个细胞占据很大的空间。6)郎格罕细胞与Thy-1阳性细胞之间有密切的分布关系。7）在鸡表皮中发现了ATP酶阳性的Langerhans样细胞。

项目成果

Koyama,Y.Hachiya,T.Hagiwara,M.,Kobayashi,M.,Hoshino,T.,hidaka,H.,and Marunouchi,T.: "Expression of protein kinase C isozyme in epidermal Langerhand cells." J.Invest.Dermatol.94. 677-680 (1990)

Koyama，Y.Hachiya，T.Hagiwara，M.，Kobayashi，M.，Hoshino，T.，hidaka，H.，和 Marunouchi，T.：“表皮 Langerhand 细胞中蛋白激酶 C 同工酶的表达。”

Hayakawa,M.,Kobayashi,M.,and Hoshino,T.: "The direct contact between the reticular fibers and migratory cells in the paracortex of mouse lymph nodes ^-a morphological and quantitative study." Arch.Histol.Cytol.51. 233-240 (1988)

Hayakawa, M.、Kobayashi, M. 和 Hoshino, T.：“小鼠淋巴结副皮质中网状纤维和迁移细胞之间的直接接触^-一项形态学和定量研究。”

Kotani,H.,Kobayashi,M.,Niwa,J.,Kaneda,T.,and Hoshino,T.: "Langerhans cells in the developing mucosal epithelium of mice." Arch.Histol.Cytol.54. (1991)

Kotani,H.、Kobayashi,M.、Niwa,J.、Kaneda,T. 和 Hoshino,T.：“小鼠发育中的粘膜上皮中的朗格汉斯细胞。”

Koyama,Y.,Kobayashi,M.,Ohashi,K.Nagano,S.,Niwa,J.,Takahashi,H.,Hoshino,T.,and Marunouchi,T.: "Isolation and culture of pannig methodーinduced Langerhans cells from Dispaseーdissociasted epidermal cells of the mouse." J.Dermatol.17. 211-217 (1990)

Koyama, Y.、Kobayashi, M.、Ohashi, K. Nagano, S.、Niwa, J.、Takahashi, H.、Hoshino, T. 和 Marunouchi, T.：“pannig 方法诱导的朗格汉斯的分离和培养来自小鼠分散酶解离表皮细胞的细胞。” J.Dermatol.17. 211-217 (1990)

Koyama,Y.et al: "Expression of protein kinase C isozyme in epidermal Langerhans cells of the mouse." J.Invest.Dermatol.印刷中. (1990)

Koyama, Y. 等人：“蛋白激酶 C 同工酶在小鼠表皮朗格汉斯细胞中的表达”，J. Invest 出版。