Intracellular behavior of a multifunctional protein, collagenase inhibitor.

应用免疫组织化学方法研究了人牙龈成纤维细胞（Gin-1细胞）和其他人细胞系中金属蛋白酶组织抑制剂的细胞内定位。TIMP-免疫染色在所有细胞的细胞质和一些细胞群的细胞核中观察到。对Gin-1细胞核和胞质免疫染色密度的定量观察表明，当细胞被从培养物中去除血清而停滞在G 0时，核密度显著降低，并且随后的血清诱导的细胞生长伴随着核TIMP阳性细胞的增加。而细胞质密度几乎保持不变，除了在G 0时略有下降。核内TIMP阳性细胞的频率与PCNA阳性细胞的频率呈平行变化，但略低于S期细胞，且始终高于S期细胞。在Raji细胞，不产生胶原，TIMP免疫染色观察到细胞质和细胞核。核TIMP-阳性细胞的频率在细胞分裂周期中发生变化。具有高增殖活性的HeLa细胞显示高频率的核TIMP阳性细胞以及PCNA阳性细胞。在各种细胞类型中，TIMP可能在支持细胞分裂周期的进展中起作用。

Intracellular locarization of tissue inhibitor of metalloproteinases was investigated in human gingival fibroblasts (Gin-l cells) and other human cell lines in culture by immunohistochemistry. The TIMP-immunostaining was observed in the cytoplasm of all cells and in the nuclei of some, population of cells. Quantitative observations on the nuclear and cytoplasmic densities of the immunostaining in Gin-1 cells demonstrated that the nuclear density was remarkably decreased when the cells were arrested at G0 by removal of serum from the culture, and the following seruminduced cell growth was accompanied by an increase of nuclear TIMP-positive cells. While the cytoplasmic density remained almost unchanged except a slight decrease at G0. The frequency of the nuclear TIMP-positive cells changed in parallel with that of PCNA-positive cells at a little lower level and was always higher than that of S-phase cells. In Raji cells, which do not produce collagen, TIMP immunostaining was observed in the cytoplasm and nucleus. The frequency of nuclear TIMP-positive cells changed during the cell division cycle. HeLa cells having a high activity of proliferation showed a high frequency of nuclear TIMP-positive cells as well as PCNA-positive cells. In various cell types TIMP might function in supporting the progression of the cell division cycle.