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Immunology and Molecular Biology of Cestode Infections.

绦虫感染的免疫学和分子生物学。

基本信息

批准号：
63480168
负责人：
ONITAKE Kazuo
金额：
$ 3.9万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

The host-parasite interaction in cestode infections has been analyzed using several species of cestodes, Hvmenolepis spp. and Taenia taeniaeformis in mice and rats. At least two different species are required as the intermediate and definitive hosts for completion of the life-cycle of cestodes except H. nana. In the present work, we have analyzed (1) immunogenicity of adult cestodes in the intestinal lumen of deinitive host with emphasis on the fate of cncospheres produced by and released from mature adult worms, and (2) that of larval cestodes in the tissue of the intermediate host with emphasis on the host-protective antigens of the oncosphere. In the former, it has become clear that (1) adult cestodes are immunogenic but the immunogenicity differs from that of larval cestodes, (2) immunity to larval cestodes or adult cestodes is stage-specific but not always species-specific. On the life cycle of cestodes, we have found tht oncosphere embryos produced by and released from adult csto … More des of H. microstoma and H. diminuta, which require beetles as the intermediate host, may hatch and invade the intestinal tissue of the definitive host. Although it is open to question whether oncospheres invaded the tissue of the definitive host could develop into larval cstodes, this observation provides a good explanation as to why mice experienced patent infecion with H. microstoma produce anti-oncosphere antibodies in addition to anti-larval and anti-oncosphere antibodies and why mice given viable eggs of this parasite produce anti-oncosphere antibodies exclusively and show strong resistance to challenge infection with oncospheres of H. nana. At present, we may speculate the reason why anti-oncosphere antibodies are produced in the definitive host dogs infected with Echinocococcus granulosus or E, multilocularis of medical importance. In the latter, we have shown the importance of oncospheres as the immunogen in mammalian intermediate hosts. With collaboration with Australian scientists, we have succeeded in establishing Escherichia coli producing oncosphere stage-specific and host-protective polypeptide of 21 kDa. This is the first demonstrating oncosphere stage-specific component produced by recombinant DNA technology. Less

已使用几种绦虫（Hvmenolepis spp.）和大鼠带绦虫感染。除H外，绦虫的生活史至少需要两种不同的中间宿主和终宿主。奶奶本工作分析了（1）成虫绦虫在致病宿主肠腔内的免疫原性，重点是蠕虫产生和释放的六钩蚴的命运;（2）幼虫绦虫在中间宿主组织中的免疫原性，重点是六钩蚴的宿主保护性抗原。在前者中，已经清楚的是（1）成虫绦虫具有免疫原性，但免疫原性不同于幼虫绦虫，（2）对幼虫绦虫或成虫绦虫的免疫具有阶段特异性，但不总是种特异性。在绦虫的生活史中，我们发现了由成虫产生和释放的六钩蚴胚胎 ...更多信息 des of H. microstoma和H.缺陷虫需要甲虫作为中间宿主，可以孵化并侵入终宿主的肠组织。虽然六钩蚴侵入终末宿主组织后能否发育成幼虫绦虫还有待商榷，但这一观察结果很好地解释了为什么小鼠会经历H. microstoma除了抗幼虫和抗六钩蚴抗体外，还产生抗六钩蚴抗体，以及为什么给予这种寄生虫的活卵的小鼠仅产生抗六钩蚴抗体，并对H.奶奶目前，我们可以推测抗六钩蚴抗体在感染细粒棘球蚴或医学上重要的多房棘球蚴的终末宿主犬中产生的原因。在后者中，我们已经表明六钩蚴作为哺乳动物中间宿主的免疫原的重要性。与澳大利亚科学家合作，我们已经成功地建立大肠杆菌生产六钩蚴阶段特异性和主机保护多肽的21 kDa。这是第一个证明六钩蚴阶段特定的组成部分产生的重组DNA技术。少