Analysis of the role of epidermal cytokines in skin inflammation

表皮细胞因子在皮肤炎症中的作用分析

• 批准号: 63480246
• 负责人: NISHIOKA Kiyoshi
• 金额: $ 2.75万
• 依托单位: Kitasato University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480246/
• 关键词: Epidermal cytokine; Psoriasis; Interleukin 1; Interleukin 6; In situ hybridization; 尋常性乾癬; 表皮サイトカイン; 乾癬; IL1; IL6; インサイチュ・ハイブリダイゼ-ション; 表皮細胞株; 表皮細胞由来胸腺細胞活性化因子(ETAF); インターロイキン1α

We have developed a method to detect messenger RNA (mRNA) expression of keratinocytes in situ in the study performed in the previous year. We have applied this technique to examine the role of interleukins in psoriasis, one of typical epidermal proliferating diseases, this year.Biopsied specimens were obtained from 27 patients with psoriasis vulgaris and 9 healthy volunteers after informed consent. They were snap-frozen in liquid nitrogen and 10 mu thick sections were prepared. The sections were fixed with 4% paraformaldehyde and ethanol, and hybridized with biotin labeled complementary DNA (cDNA) of interleukin (IL) -1, IL-6 and IL-6 receptor (IL-6R). They were treated with avidin alkaline phosphatase and its enzyme substrates.IL-1 message was expressed by healthy skin, uninvolved skin of psoriasis and transitional zone between uninvolved and fully developed psoriatic lesion but not in psoriatic lesion. The message of IL-6 was detected in the uninvolved, transitional zone and psoriatic lesion but not in healthy skin. It was expressed more strongly in transitional zone and tended to fade in psoriatic lesion. IL-6R message was found in uninvolved and transitional zone but not in psoriatic lesion and healthy skin.Thus synthesis of IL-6 and its receptor was regulated in the psoriatic skin by certain factors to suppress excess proliferation as is found in neoplasmas. IL-6 and its receptor expression by psoriatic skin play an important role in pathogenesis of psoriasis.

在前一年的研究中，我们开发了一种原位检测角质形成细胞信使RNA(MRNA)表达的方法。今年，我们应用这项技术检测了白介素性银屑病在银屑病中的作用，这是一种典型的表皮增生性疾病。将它们在液氮中快速冷冻，制备10亩厚的切片。切片用4%多聚甲醛和乙醇固定，与生物素标记的白介素1、白介素6和白介素6受体的互补DNA杂交。IL-1在正常皮肤、银屑病非皮损皮肤和银屑病皮损与完全皮损之间的过渡区表达，但在银屑病皮损中不表达。在正常皮肤、移行区和银屑病皮损中检测到IL-6的表达，而在正常皮肤中未检测到。在移行区表达较强，在银屑病皮损中表达减弱。IL-6R在非皮损区和移行区表达，但在银屑病皮损和正常皮肤中不表达，因此，银屑病患者皮肤中IL-6及其受体的合成受某些因子的调节，从而抑制过度增殖，与肿瘤一样。银屑病患者皮肤中IL-6及其受体的表达在银屑病发病机制中起重要作用。

项目成果

K.Nishioka,;I.Katayama,;H.ETO,;Y.Sato,;T.Funato,: Dermatologica. 1989.

K.Nishioka,;I.Katayama,;H.ETO,;Y.Sato,;T.Funato,：皮肤病学。

Nishioka K, Funato T, Sato Y, Eto H, Katayama I and Nishiyama S: "Production of interleukin 1 alpha by a trichilemmoma cell line." Journal of Dermatology 1990.

Nishioka K、Funato T、Sato Y、Eto H、Katayama I 和 Nishiyama S：“毛毛虫瘤细胞系产生白细胞介素 1 α”。

Nishioka k,katayama I,ETo,H,Funato T: "Trichilemmona cell live can qeuevate interleukin 1 alpha" Dermatologica. 179. 135-176 (1989)

Nishioka k,katayama I,ETo,H,Funato T：“Trichilemmona 细胞活体可以使白细胞介素 1 α 升高”Dermatologica。

Nishioka K,Funato T,Sato Y,Eto H,Katayama I,Nishiyama S.: "Production of interleukin 1 alpha by a trichilemmoma cell line" Journal of Dermatology. (1990)

Nishioka K、Funato T、Sato Y、Eto H、Katayama I、Nishiyama S.：“毛毛虫瘤细胞系产生白细胞介素 1 α”皮肤病学杂志。

Nishioka K,Funato T,Sato Y,Ohta Y.Eto H.Katayama I,Nishiyama S: "Droduction of interleukin 1 alpha by a trichilemmoma cell live" Journal of Deymatocepy(印刷中). (1990)

Nishioka K、Funato T、Sato Y、Ohta Y. Eto H. Katayama I、Nishiyama S：“毛毛虫细胞活体产生白介素 1 α”Journal of Deymatocepy（出版中）（1990 年）。