Integrative and trans-ethnic study to understand psoriasis associated signals

项目总结 牛皮癣是一种慢性免疫调节的皮肤病，对公众健康有重大影响，每年 在美国，直接和间接成本超过750亿美元。高通量技术的进步已经 能够识别Th17/IL-23和NF𝜅B轴上的遗传和基因组成分，相关 患有牛皮癣病理。虽然已经发现了80个牛皮癣易感地区，但有相当大的 缩小导致遗传变异的范围并弄清其病理机制仍然是一个挑战。 形成了疾病病因学。同样，尽管核因子𝜅B信号参与了银屑病和不同皮肤的免疫 作为一种疾病，我们对遗传变异在核因子𝜅B调控中所起的机制作用的了解非常有限。 随着新的扩张型银屑病的出现，银屑病可以作为一种理想的皮肤病模型来研究 角质形成细胞中的这种现象。牛皮癣在非裔美国人(AA)中的患病率为1.3%， 然而，大多数美国建立的银屑病基因组学研究都是在欧洲美洲人(EA)上进行的。 人口。我们的初步数据表明，组件的精细映射可以通过集成 多种族分析设计中的遗传、表观遗传和基因组信息，特别是当包括 具有非洲血统的个人。我们已经展示了角质形成细胞中高水平的核因子𝜅B信号反应 再生障碍性贫血患者和炎性征象的个体间差异可能具有显著的临床意义 对药物反应评估的影响。这个项目的长期目标是识别生物 银屑病患者疾病异质性的机制，我们的总体目标是利用一种反式- 基因设计以促进银屑病相关调控机制的识别 角质形成细胞中核因子𝜅B信号的调控。我们将采用综合的方法来研究多组学数据 并利用跨种族信息精细绘制与种族间相关的遗传/基因组成分图 个体炎症反应，为了解银屑病患者之间的疾病差异提供了一个模型 不同种族的人。我们将1)精细定位调控反应表达的数量性状基因座(reQTL 角质形成细胞中的核因子𝜅B和其他炎症信号；II)确定银屑病信号的调节机制 利用多模式基因组数据在细胞水平上驱动角质形成细胞中的NF𝜅B信号；iii)利用基因 和参与NF𝜅B信号转导的基因组组件，以模拟临床表现和预后 来自持续的纵向牛皮癣队列的患者。该项目的成功完成将有一个 通过提供更高的分辨率和能力来确定个人间的决定因素，从而产生重要的积极影响 炎症反应的变化。

PROJECT SUMMARY Psoriasis is a chronic immune-mediated skin disease that has a significant impact on public health, with annual direct and indirect costs over $75 billion dollars in the US. Advancements in high throughput technology have enabled the identification of genetic and genomic components in the Th17/IL-23 and NF𝜅B axes, associated with psoriasis pathology. Although >80 psoriasis susceptibility regions have been revealed, considerable challenges remain in narrowing down the causal genetic variations and discerning their pathological mechanisms that shape disease etiology. Similarly, while NF𝜅B signaling is involved in psoriasis and different skin immune disorders, we have very limited understanding of the mechanistic role genetic variants play in NF𝜅B regulation. With the new extended psoriasis GWAS emerging, psoriasis can serve as an ideal skin disease model to study this phenomenon in keratinocytes. Psoriasis has a prevalence rate of 1.3% among African Americans (AA), however most US-established genomics studies of psoriasis have been conducted on European American (EA) populations. Our preliminary data show that the fine-mapping of components can be facilitated by integrating genetic, epigenetic, and genomic information in a multi-ethnic analysis design, especially when including individuals with African ancestry. We have illustrated elevated NF𝜅B signaling response in keratinocytes among AA individuals and that inter-individual variations in inflammatory signature can have significant clinical implications for the assessment of drug response. The long-term goal of this project is to identify biological mechanisms for disease heterogeneity among psoriatic patients, and our overall objective is to utilize a trans- ethnic design to advance the identification of psoriasis-associated regulatory mechanisms involved in the modulation of NF𝜅B signaling in keratinocytes. We will apply an integrative approach to study multi-omics data and leverage trans-ethnic information to fine-map the genetic/genomic components associated with inter- individual inflammatory responses, providing a model to understand disease disparity among psoriatic patients of different ethnicities. We will i) fine-map the response expression quantitative trait loci (reQTLs) modulating NF𝜅B and other inflammatory signaling in keratinocytes; ii) determine regulatory mechanisms of psoriasis signal that drive NF𝜅B signaling in keratinocytes at the cellular level using multi-modal genomic data; iii) utilize genetic and genomic components participating in NF𝜅B signaling, to model clinical presentations and outcomes for patients from an ongoing longitudinal psoriasis cohort. Successful completion of the project will have an important positive impact by providing enhanced resolution and power to identify determinants of inter-individual variations in inflammatory responses.