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Synthesis of Carbohydrate Complex and its Significant Role in the Vestibular Specialized Cells

碳水化合物复合物的合成及其在前庭特化细胞中的重要作用

基本信息

批准号：
63480380
负责人：
HARADA Yasuo
金额：
$ 1.09万
依托单位：
Hiroshima University (1989-1990)Kyushu University (1988)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

The synthesizing process of the carbohydrate complex and its significant role in the vestibular end organs were investigated in order to reveal the early changes in the vestibular functional disorders. The surface of the vestibular sensory epithelia including sensory, supporting, transitional and dark cells, was covered by fuzzy layers of glycocalyx. It has been indicated that the significant role of the glycocalyx is to keep the cilia arranged in a bundle, to be involved in the sensory cell transduction system, to be related with the controlling mechanisms of the ionic environment of the endolymph and to play an important role for the protection of the supporting cells. The ultrastructural localization of the carbohydrate complexes were also demonstrated using gold-labelled lectins. It has been indicated that the glycocalyx and its associated structures contain N-acetyl-glucosamine, galactose, mannose and sialic acid. These sugars may thus be closely related to the different function of the glycocalyx. In the pathological conditions such as after ototoxic drug administration, endolymphatic hydrops, acoustic overstimulation etc, the first step of degeneration process in the vestibular sensory epithelia was a decrease in glycocalyx or changes of carbohydrate complexes. The further degeneration such as fusion, disarrangement and disappearance of the cilia were all occurred as a result of the damage of glycocalyx.

本研究旨在探讨前庭终器糖复合物的合成过程及其在前庭终器中的重要作用，以揭示前庭功能障碍的早期变化。前庭感觉上皮包括感觉细胞、支持细胞、移行细胞和暗细胞，其表面覆盖着模糊的糖萼层。研究表明，糖萼的重要作用是维持纤毛成束排列，参与感觉细胞的信号转导系统，与内淋巴离子环境的调控机制有关，并对支持细胞的保护起重要作用。的碳水化合物复合物的超微结构定位也证明了使用金标记凝集素。已表明糖萼及其相关结构含有N-乙酰氨基葡萄糖、半乳糖、甘露糖和唾液酸。因此，这些糖可能与糖萼的不同功能密切相关。在耳毒性药物、内淋巴积水、声过度刺激等病理条件下，前庭感觉上皮细胞的变性过程首先是糖萼减少或糖复合物改变。纤毛进一步变性，如融合、排列紊乱和消失，都是糖萼损伤的结果。