Calcium Metabolism in the Yestibular Non-Sensory Cells.

本文对钙在前庭黄斑细胞中的作用进行了研究。耳蜗的合成不仅与分泌颗粒有关，还与支持细胞的溶酶体和高尔基体有关。硫酸Steptomycin抑制溶酶体酶活性，导致钙分泌减少。这可能会导致耳聋的减少。通过X射线微量分析仪的研究，证实耳石被前庭暗细胞吸收，提示硫酸链霉素影响了暗细胞的功能。钙离子在离体前庭感觉细胞内的流动可以被证实。在去极化状态下，钙离子快速流入细胞，而在静息状态下，钙离子的分布没有特定的规律。内淋巴上皮细胞中存在由光细胞受体激活的钙通道，调节内淋巴。用扫描电镜观察皂素灌注法可以观察细胞骨架的三维结构以及与各种膜结合细胞器如细胞核、线粒体、内质网、高尔基体等相互作用的复合物。分离的活前庭感觉细胞能够产生自我运动。肌动蛋白被认为与前庭毛细胞的运动有关。提示前庭毛细胞的运动与肌动蛋白-肌球蛋白系统和离子通过质膜的转运有关。我相信这一系列关于前庭黄斑细胞钙的研究提供了许多有用的信息。

The following results of the functions of calcium in the cells of vestibular maculae were obtained.Not only the secretory granules but also lysosomes and Golgi apparatus of supporting cells were concerned with the synthesis of otoconia. Steptomycin sulfate inhibited the lysosomal enzyme activity as a result the secretion of calcium were decreased. This might lead to reduction of otoconia. Studying by X ray microanalyzer, it was certained that otoconia was absorbed into vestibular dark cells and it was suggested that streptomycin sulfate affected the functions of dark cells.Calcium ion flow in the isolated vestibular sensory cells could be demonstrated. Rapid flow of calcium ion into the cells during the depolarized state was observed while calcium distribution had no particular patterns during the resting state. There were the calcium channels whici were activated by the receptor of light cells in endolymphatic epithelial cells which regulated endolymph.Saponin perfusion method using scanning electron microscopy made it possible to investigate three-demensional structures of cytoskeletons as well as the complex interacted with various membranous bound organelles such as nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus and so on.Isolated living vestibular sensory cells were capable of producing self movement. Actin is thought to be related to vestibular hair cell motility. It was suggested that the motility of vestibular hair cells depends on the actin-myosin system and the ion transport through the plasmic membrane.I believe that this series of studies on calcium in the cells of vestibular maculae presents many useful informations.