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Studies in sunaptic vesicles of the sensory cells and the nerve endings of the inner ear

感觉细胞的日触囊泡和内耳神经末梢的研究

基本信息

批准号：
60480381
负责人：
HARADA Yasuo
金额：
$ 0.77万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

Intracellular structures of the inner ear were observed by scanning electron microscopy for the studies in synaptic vesicles. Macula, crista ampullaris and cochlear were freeze fractured and then macerated with 0.1% OsS4 solution (aldehyde-Osmium-DMSO-Osmium method). Intracellular organells such as mitochondria, endoplasmic reticulum (ER) and Golgi apparatus were demonstrated stereoscopically. Moreover, the structural characteristic of the nerve endings (efferent and afferent nervr endings) was demonstrated and the relation of synaptic bars and synaptic vesicles was morphologically shown. Especially the complicated networks of the tubular ER of a cochlear outer hair cell had an interesting structure in regard to the mechanoelectrical transduction to afferent neurons.Physiolosically the action potential from afferent neruons of the vestibular organ of a bull frog was recorded with a glass electrode and the characteristics of the outer hair cell's membrane of guinea pig were studied using the patch clamp method. According to the pattern of the action potential there were the physiological localization of the receptors in the crista and polarity of the utricular macula. On the one hand, the single outer hair cell of a guinea pig was used in the patch clamp experiment. Membrane currents consisting of the initial inward and the following outward current were obtained by the whole cell recording of the patch clamp method. It was assumed that this inward current was caused by calcium ion which was an important ion in discharge of the chemical transmitters to the sunaptic cleft.These results were new findings and seemed to be a stepping stone to the further studies in the inner ear study.

用扫描电镜观察内耳细胞内结构，研究突触囊泡。将黄斑、壶腹嵴和耳蜗冷冻断裂后用0.1%的OsS 4溶液浸渍（Crude-Osmium-DMSO-Osmium法）。立体显示了细胞内的线粒体、内质网和高尔基体等细胞器。此外，还从形态学上揭示了神经末梢（传出和传入神经末梢）的结构特征，以及突触棒和突触小泡的关系。用玻璃电极记录牛蛙前庭器官传入神经元动作电位，并用膜片钳技术研究豚鼠耳蜗外毛细胞膜的特性。根据动作电位的类型，在椭圆囊斑的嵴和极性上存在受体的生理定位。一方面，在膜片钳实验中使用豚鼠的单个外毛细胞。用膜片钳全细胞记录法记录细胞膜电流，包括初始内向电流和随后的外向电流。推测这种内向电流是由钙离子引起的，钙离子是化学递质向视丘间隙放电的重要离子，这些结果是新的发现，为进一步研究内耳结构奠定了基础。