Monoclonal antibodies to the neoplasic epithelial duct cell of human salivary gland origin: Production and application to immunohistochemy of salivary gland tumors

人唾液腺肿瘤上皮导管细胞单克隆抗体的制备及其在唾液腺肿瘤免疫组化中的应用

• 批准号: 63480403
• 负责人: YAGI Toshio
• 金额: $ 3.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480403/
• 关键词: Salivary gland tumor; Monoclonal antibody; Hybridoma; Immunohistochemistry; Enzyme-linked immunosorbent assay; Western blotting; モノクローナル抗体

Ninety monoclonal antibodies (MoAbs) were obtained after immunization of mouse neoplastic epithelial cells of human salivary gland origin (HSG). Twenty five MoAbs were applicable to immunohistochemistry. Based on the immunoreactivity with normal salivary gland tissue (parotid gland and submandibular gland), three reaction types were recognized, i.e., type 1: reactive with whole epithelial elements. type 2: reactive with the terminal epithelal elements (acinus and intercalated duct) and type 3: reactive with the nonterminal epithelial elements (striated duct and excretory duct). Seven MoAbs showed type 1 reactivity, four type 1 or 2, eleven type 2, and three type 3. None of the MoAbs reacted with the mucous acinar cells in submandibular gland. Though the parenchymal cells of the duct-like component in pleomorphic adenoma was positive for all types of MoAbs, those of sheath-like component around duct and solid cellular component were never reactive with type 3 MoAbs. Both myxoid and chondroid components were almost negative for all types of Mo Abs. Warthin's tumor was negative for type 2 MoAbs and adenoid cystic carcinoma was negative for type 3 MoAbs. The epidermoid cells in mucoepidermoid tumor were positive for both type 1 and type 2 MoAbs, whereas, the mucus-secreting cells were positive for type 3 MoAbs. These results show that the anti-HSG MoAbs are useful tools for the analysis of histogenesis of salivary glind tumor. Moreover, these MoAbs are also usable for classification of salivary gland tumors. Analyses of antigens recognized by MoAbs were carried out. Nine MoAbs recognized the secretory products of HSG and ten recognized the nonsecretory ones. Western blotting revealed that all MoAbs recognized the antigens with molecular weight of 35.4 Kd and 30.6 Kd under reduced state.

用人涎腺源性肿瘤上皮细胞（HSG）免疫小鼠，获得90株单克隆抗体（MoAbs）。25株单抗可用于免疫组化。基于与正常唾液腺组织（腮腺和下颌下腺）的免疫反应性，识别出三种反应类型，即，1型：与整个上皮成分反应。第二类：与末端上皮成分（腺泡和闰管）反应，3型：与非末端上皮成分（纹状管和排泄管）反应。7种单克隆抗体显示1型反应性，4种1型或2型，11种2型和3种3型。所有单克隆抗体均不与颌下腺粘液腺泡细胞发生反应。多形性腺瘤中导管样成分的实质细胞对所有类型的MoAb均呈阳性反应，而导管周围的鞘样成分和实性细胞成分与3型MoAb均不反应。粘液样和软骨样成分几乎为阴性的所有类型的钼抗体。Warthin肿瘤对2型MoAb呈阴性，腺样囊性癌对3型MoAb呈阴性。粘液表皮样瘤的表皮样细胞对1型和2型MoAb均呈阳性反应，而粘液分泌细胞对3型MoAb呈阳性反应。这些结果表明，抗HSG单克隆抗体是分析涎腺胶质瘤组织发生的有用工具。此外，这些单克隆抗体也可用于唾液腺肿瘤的分类。对单克隆抗体识别的抗原进行了分析。9个单克隆抗体识别HSG的分泌产物，10个单克隆抗体识别非分泌产物。Westernblotting结果显示，所有单抗在还原状态下均能识别分子量为35.4Kd和30.6Kd的抗原。