Monoclonal antibodies to the neoplasic epithelial duct cell of human salivary gland origin: Production and application to immunohistochemy of salivary gland tumors

用人涎腺来源的小鼠肿瘤上皮细胞(HSG)免疫小鼠，获得90株单抗(MoAbs)。25株MoAbs可用于免疫组织化学。根据对正常唾液腺组织(腮腺和颌下腺)的免疫反应，可识别出三种反应类型，即类型1：与全部上皮细胞反应。类型2：与终末上皮细胞(腺泡和间管)反应；类型3：与非终末上皮细胞反应(纹状管和排泄管)。7株MoAb呈1型反应，4株呈1型或2型，11株呈2型，3株呈3型。未见与颌下腺粘液腺泡细胞反应。尽管多形性腺瘤管样成分的实质细胞对所有类型的MoAbs均呈阳性反应，但导管周围的鞘状成分和实性细胞成分的实质细胞与3型MoAbs均不反应。粘液和软骨样成分对所有类型的Mo Abs几乎均为阴性。沃辛瘤为阴性，腺样囊性癌为阴性。粘液表皮样瘤中的表皮样细胞同时表达1型和2型MoAbs，而分泌粘液的细胞表达3型MoAbs。这些结果表明，抗HSG MoAbs是分析涎腺闪光性肿瘤组织发生的有用工具。此外，这些MoAbs还可用于涎腺肿瘤的分类。对MoAbs识别的抗原进行了分析。9株抗HSG抗体识别HSG分泌性产物，10株识别HSG非分泌性产物。Western blotting结果表明，所有MoAbs均能识别相对分子质量分别为35.4kD和30.6kD的抗原。

Ninety monoclonal antibodies (MoAbs) were obtained after immunization of mouse neoplastic epithelial cells of human salivary gland origin (HSG). Twenty five MoAbs were applicable to immunohistochemistry. Based on the immunoreactivity with normal salivary gland tissue (parotid gland and submandibular gland), three reaction types were recognized, i.e., type 1: reactive with whole epithelial elements. type 2: reactive with the terminal epithelal elements (acinus and intercalated duct) and type 3: reactive with the nonterminal epithelial elements (striated duct and excretory duct). Seven MoAbs showed type 1 reactivity, four type 1 or 2, eleven type 2, and three type 3. None of the MoAbs reacted with the mucous acinar cells in submandibular gland. Though the parenchymal cells of the duct-like component in pleomorphic adenoma was positive for all types of MoAbs, those of sheath-like component around duct and solid cellular component were never reactive with type 3 MoAbs. Both myxoid and chondroid components were almost negative for all types of Mo Abs. Warthin's tumor was negative for type 2 MoAbs and adenoid cystic carcinoma was negative for type 3 MoAbs. The epidermoid cells in mucoepidermoid tumor were positive for both type 1 and type 2 MoAbs, whereas, the mucus-secreting cells were positive for type 3 MoAbs. These results show that the anti-HSG MoAbs are useful tools for the analysis of histogenesis of salivary glind tumor. Moreover, these MoAbs are also usable for classification of salivary gland tumors. Analyses of antigens recognized by MoAbs were carried out. Nine MoAbs recognized the secretory products of HSG and ten recognized the nonsecretory ones. Western blotting revealed that all MoAbs recognized the antigens with molecular weight of 35.4 Kd and 30.6 Kd under reduced state.