Monoclonal antibodies to the neoplasic epithelial duct cell of human salivary gland origin: Production and application to immunohistochemy of salivary gland tumors

用人唾液腺源性小鼠肿瘤上皮细胞（HSG）免疫获得90个单克隆抗体（MoAbs）。25种moab抗体适用于免疫组织化学。根据与正常唾液腺组织（腮腺和颌下腺）的免疫反应性，可识别出三种反应类型，即1型：与整个上皮细胞反应。2型：与末端上皮细胞（腺泡和插管）反应，3型：与非末端上皮细胞（横纹管和排泄管）反应。1型反应性7种，1或2型4种，2型11种，3型3种。MoAbs与颌下腺粘液腺泡细胞均无反应。多形性腺瘤的导管样实质细胞对3型moab均阳性，而导管周围鞘样实质细胞和实体细胞对3型moab均无反应。黏液和软骨样成分对所有类型的MoAbs几乎都是阴性的。Warthin的肿瘤对2型MoAbs是阴性的，腺样囊性癌对3型MoAbs是阴性的。黏液表皮样瘤的表皮样细胞对1型和2型moab抗体均呈阳性，而黏液分泌细胞对3型moab抗体呈阳性。这些结果表明，抗hsg单克隆抗体是分析涎腺肿瘤组织发生的有效工具。此外，这些moab也可用于唾液腺肿瘤的分类。对MoAbs识别的抗原进行分析。9个moab识别HSG的分泌产物，10个识别非分泌产物。Western blotting结果显示，在还原状态下，moab均能识别分子量为35.4 Kd和30.6 Kd的抗原。

Ninety monoclonal antibodies (MoAbs) were obtained after immunization of mouse neoplastic epithelial cells of human salivary gland origin (HSG). Twenty five MoAbs were applicable to immunohistochemistry. Based on the immunoreactivity with normal salivary gland tissue (parotid gland and submandibular gland), three reaction types were recognized, i.e., type 1: reactive with whole epithelial elements. type 2: reactive with the terminal epithelal elements (acinus and intercalated duct) and type 3: reactive with the nonterminal epithelial elements (striated duct and excretory duct). Seven MoAbs showed type 1 reactivity, four type 1 or 2, eleven type 2, and three type 3. None of the MoAbs reacted with the mucous acinar cells in submandibular gland. Though the parenchymal cells of the duct-like component in pleomorphic adenoma was positive for all types of MoAbs, those of sheath-like component around duct and solid cellular component were never reactive with type 3 MoAbs. Both myxoid and chondroid components were almost negative for all types of Mo Abs. Warthin's tumor was negative for type 2 MoAbs and adenoid cystic carcinoma was negative for type 3 MoAbs. The epidermoid cells in mucoepidermoid tumor were positive for both type 1 and type 2 MoAbs, whereas, the mucus-secreting cells were positive for type 3 MoAbs. These results show that the anti-HSG MoAbs are useful tools for the analysis of histogenesis of salivary glind tumor. Moreover, these MoAbs are also usable for classification of salivary gland tumors. Analyses of antigens recognized by MoAbs were carried out. Nine MoAbs recognized the secretory products of HSG and ten recognized the nonsecretory ones. Western blotting revealed that all MoAbs recognized the antigens with molecular weight of 35.4 Kd and 30.6 Kd under reduced state.