Investigation of the Pharmacological Effect of Calcium Hydroxide with a Rabbit Ear Chamber Technique

兔耳室技术研究氢氧化钙的药理作用

• 批准号: 63480424
• 负责人: WAKABAYASHI Hajime
• 金额: $ 1.92万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480424/
• 关键词: Rabbit Ear Chamber; Calcium Hydroxide; Dystrofic Calcification; X-RAY Microanalyzer; IN-VIVO Observation; Root canal sealer; Microcirculation; 生体観察; ラビットイアーチェンバー法; 組織刺激

An improved rabbit ear chamber (REC) was used to investigate the dystrophic calcification after Ca(OH)_2 application on connective tissues. Ca(OH)_2 with saline was inserted into the REC and the change in microcirculation inside was observed under the vital-microscope with passage of time. Several minutes after the application, microvessels within the close vicinity to Ca(OH)_2 began to disappear. After 3 hours, at the border between the living tissue and liquefacted area, many precipitates were observed. They became large in both size and number to combine like a bank until 48 hours. SEM and EDX investigation revealed that these precipitates contained Ca, P, S,and Mg and they were produced by neutralization of Ca(OH)_2 with the tissue fluid. These precipitate bank had a high affinity with the microcirculation as long as 14 weeks. The tissue-side of the bank became a plate-like form, absorbing Ca and P from the tissue fluid. It seems that the bank affords a bandage for the exposed pulp tissue to begin the deposition of reparative dentine. The characteristic leature of the pharmacological effect of Ca(OH)_2 may be to make such a neutralized precipitates that introduces dystrophic calcification.Therefore, the high alkalizing property and necrotized-tissue producing effect of Ca(OH)_2 were inevitable to make these precipitates, whereas these properties were thought to be negative. The Ca(OH)_2-containing root canal sealers, "Sealapex" and "Calvital", and the pulp capping agent "Dycal" produced the same precipitates. These medicine have all high alkalinity. However, the newly-developed root canal sealer, which constituents were Ca(OH)_2 and fatty acid, was neutral in pH and did not produce such precipitates. It need further experiments to determine which is a bette property as an endodontic materials, but we found out that there were two different types in so-called Ca(OH)_2-containing root canal sealers.

本文用改良兔耳室（REC）观察Ca（OH）_2作用于结缔组织后的营养不良性钙化。将Ca（OH）_2与生理盐水一起插入REC中，在活体显微镜下观察REC内微循环随时间的变化。应用后数分钟，Ca（OH）_2附近的微血管开始消失。3小时后，在活组织和坏死区域之间的边界处，观察到许多沉淀物。他们在规模和数量上都变得很大，像银行一样联合收割机，直到48小时。扫描电镜和能谱分析表明，这些沉淀物中含有Ca、P、S和Mg，它们是由Ca（OH）_2与组织液中和而成。这些沉淀物库与微循环有很高的亲和性，长达14周。组织侧的银行成为一个板状的形式，从组织液中吸收钙和磷。看来，银行提供了一个绷带，为暴露的牙髓组织开始沉积修复牙本质。Ca（OH）_2药理作用的特征可能是产生一种中和沉淀，引起营养不良性钙化，因此Ca（OH）_2的强碱化性和坏死组织生成作用是产生这些沉淀的必然，而这些性质被认为是负面的。含Ca（OH）_2的根管封闭剂Sealapex和Calvital与盖髓剂Dycal产生相同的沉淀。这些药都是高碱性的。而新研制的根管充填剂，其成分为Ca（OH）_2和脂肪酸，pH为中性，不产生上述沉淀。但我们发现，含Ca（OH）_2的根管封闭剂有两种不同的类型，即Ca（OH）_2-Ca（OH）_2-Ca（OH）_2-Ca（OH）_2-Ca（OH）_2）_2-Ca（OH）_2）_2-Ca（OH）_2）_2。

项目成果

H. WAKABAYASHI, A. FUNATO, K. KAMATAKI & K. MATSUMOTO: "Modification of Rabbit Ear Chamber for IN-VIVO Observation of the Irritation Potential of a Root Canal Sealer" Microcirculation Annual. 1989. 25-26 (1989)

H. 若林、A. 船户、K. Kamataki

若林 始,船登 章嘉,都築 延乏,堀川 真奈美,鎌滝 貴世子,五日月 秀行,松本 光吉: "根管充填剤の組織反応を検討するための改良型ラビットイヤ-チェンバ-について" 日本歯科保存学雑誌. 32. 1109-1114 (1989)

Hajime Wakabayashi、Akiyoshi Funato、Noboru Tsuzuki、Manami Horikawa、Kiyoko Kamataki、Hideyuki Itkazuki、Mitsuyoshi Matsumoto：“关于用于检查根管填充材料的组织反应的改进兔耳室”日本牙科诊所保护研究杂志 32. 1109。 -1114 (1989)

若林 始,堀川 真奈美,船登 章嘉,松本 光吉: "Rabbit Ear Chamber法による水酸化カルシウムの異所性石灰化に関する研究" 日本歯科保存学雑誌. 34. (1991)

Hajime Wakabayashi、Manami Horikawa、Akiyoshi Funato、Mitsuyoshi Matsumoto：“使用兔耳室法研究氢氧化钙异位钙化”，日本保守牙科杂志 34。（1991）

若林始,堀川真奈美他: "ラビットイア-チェンバ-法による根管充填剤の組織反応の検討 1.新開発根管充填剤" 日本歯科保存学雑誌.

Hajime Wakabayashi、Manami Horikawa等人：“使用兔耳室法研究根管充填材料的组织反应1。新开发的根管充填材料”日本保守牙科杂志。

H. WAKABAYASHI, A. FUNATO, T. TUZUKI, M. HORIKAWA, K. KAMATAKI, H. GOMYOH & K. MATSUMOTO: "Modification of Rabbit Ear Chamber for IN-VIVO Observation of the Irritation Potential of a Root Canal Sealer" Japanese Journal of Conservative Dentistry. vol. 32.

H.若林、A.船户、T.TUZUKI、M.堀川、K.镰田、H.GOMYOH