The healing effect of calcium hydroxide on pulp and periodontal tissue-a study on cell behavior

氢氧化钙对牙髓及牙周组织的愈合作用——细胞行为研究

• 批准号: 10671788
• 负责人: YAMAKI Keiko
• 金额: $ 2.11万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671788/
• 关键词: Dental pulp; Hard tissue formation; Calcium hydroxide; Cultured-cell; 歯随

Calcium hydroxide has been widely used in endodontic therapy such as pulp capping and root canal treatment. Although it is histopathologically demonstrated that calcium hydroxide induces the formation of hard tissue at pulp wound surface or root end, the mechanism of calcium hydroxide activity in the mineralization process is yet unclear. The purpose of this study was to generate in vitro mineralization by applying calcium hydroxide to cells in culture collected from either dental pulp or periodontal ligament, and then to figure out the effect of calcium hydroxide on hard tissue formation in vivo.The dental pulp cells were obtained from calf lower incisors. After cracking the teeth, the whole pulp was removed and dissected into incisal, cervical and radicular sections. Each section was further divided into small pieces and cultured in modified Eagle's medium supplemented with 10% fetal bovine serum to determine the most suitable portion for the experiment that is rich with cells capable of mineralization. The proliferated cells in the primary culture showed various forms indicating several cell lines. Also there was a difference in the cell composition by the harvest site. However in later passages, not all type of the cells was kept and the attempt to establish some purified cell lines ended in failure.Human periodontal ligament cells were collected from extracted teeth due to orthodontic reasons. Small pieces from the mid-third of the roots were cultured in the same manner as the bovine dental pulp. As the cells in later subcultures lost the features seen in the primary culture, simulation of in vitro mineralization process in culture system could not be achieved in this project.Further tests will be required to develop a cell-culture system similar to those seen in dental pulp or periodontal ligament in function. Administration of calcium hydroxide through a small tube in such system would probe into its role in mineralization.

氢氧化钙在牙髓盖盖、根管治疗等根管治疗中有着广泛的应用。虽然组织病理学证明氢氧化钙诱导髓创面或根端硬组织的形成，但氢氧化钙活性在矿化过程中的机制尚不清楚。本研究的目的是通过将氢氧化钙作用于牙髓或牙周韧带的培养细胞，在体外产生矿化，并研究氢氧化钙对体内硬组织形成的影响。牙髓细胞取自小腿下门牙。牙齿开裂后，将整个牙髓取出，分成切牙、颈牙和根牙三段。每个切片进一步分成小块，在添加10%胎牛血清的改良Eagle培养基中培养，以确定富含矿化细胞的最适合实验的部分。原代培养中增殖的细胞形态多样，显示出多种细胞系。不同收获地点的细胞组成也不同。然而，在后来的传代中，并不是所有类型的细胞都被保留下来，并且建立一些纯化细胞系的尝试以失败告终。从因正畸原因拔出的牙齿中收集人牙周韧带细胞。用与牛牙髓相同的方法培养根中三分之一的小块。由于后期传代培养的细胞失去了原代培养的特征，本项目无法模拟培养系统中体外矿化过程。需要进一步的试验来开发类似于在牙髓或牙周韧带中看到的功能的细胞培养系统。在这种系统中，通过一个小管给药氢氧化钙将探讨其在矿化中的作用。