Establlishment of the biochemical diagnosis of the temporomandibular joint diseases by the analysis of the tissue of the temporomandibular joint disks and synovial fluid.

通过分析颞下颌关节盘组织和滑液建立颞下颌关节疾病的生化诊断。

基本信息

批准号：
63480440
负责人：
KINO Kohji
金额：
$ 2.69万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Biochemical studies on temporomandibular joint disks (TMJ) and synovial fluid are rather rare compared with those in the orthopedic field. In the orthpedic, several biochemical studies make an important contribution to the diagnosis and treatment of the joint diseases. So, to establish the new diagnostic methods of the TMJ diseases biochemically, the authors attempted to analyze human and porcine TMJ disks and human pathologic synovial fluid.Synovial fluid was collected from upper joint cavity, and centrifuged at 3,000rpm for 10 min, and suprenatant was obtained for analysis. Proteoglycans were extracted from disks with 4M guanidin HCI. For biochemical analysis, SDS-electrophorosis, immunoelectrophorosis, gel filtration, ion exchanged chromatography, cellurose acetete strip electrophorosis, some enzyme digestions, etc, were used properly.The result were as follows : Average of total protein amounts was 1,259 g/ml in the pathologic synovial fluid, in contrast to 205 g/ml in the normal. … More Albumin appeared in normal fluid, but albumin, transferrin and IgG were found in the pathologic fluid by the immunoelectrophorosis. A/G ratio of pathologic synovial fluid was 2.26 which was close to that of serum (1.2-2.1). A protein of 78K in molecular weight, which was not found in normal synovial fluid or serum, appeared in pathologic synovial fluid in the SDS-electrophorosis. This 78K protein had a tendency to appear when there were pathologic changes in upper joint cavity (84%). From the results of clinical examination, these cases without 78K protein had a tendency to have chronic or fixed symptoms. By statistic analysis, corellation coefficient between the appearance of 78K protein and incidence of disk deformity was 0.58. This result indicated that disk deformity may have some relation to 78K protein appearance.In the pathologic synovial fluid, the glycosaminoglycans (GAG) was composed of hyaluronic acid (HA) only by the analysis of the cellulose acetate strip electrophorosis. As the GAG chains, human TMJ disks contained mainly chondroitin sulphate, and in the others dermatan sulphate and HA, but contrastively porcine TMJ disks mainly dermatan sulphate, and a few chondroitin sulphate and HA. Less

与骨科场中的颞下颌关节盘（TMJ）和滑液的生化研究相比很少见。在矫正器中，一些生化研究为关节疾病的诊断和治疗做出了重要贡献。因此，为了在生物化学上建立TMJ疾病的新诊断方法，作者试图分析人类和猪TMJ磁盘和人类病理滑液。从上关节腔收集了辅助流体，并在3,000rpm的上离心10分钟，并获得了10分钟的离心液，并获得了超级液体，并获得了分析。用4M鸟根HCI从磁盘中提取蛋白聚糖。为了生化分析，适当使用了SDS-电噬菌病，免疫噬菌病，凝胶过滤，交换色谱法，铜乙酸乙酸根电疾病，某些酶消化等。结果如下：在病理滑液中，总蛋白质量的平均值为1,259 g/ml，而正常水平为205 g/ml。 …更多的白蛋白出现在正常液体中，但是通过免疫电交易的病理液中发现了白蛋白，转铁蛋白和IgG。病理滑液的A/G比为2.26，接近血清（1.2-2.1）。在正常滑液或血清中未发现的78K分子量的蛋白质出现在SDS-电动噬菌病的病理滑液中。当上关节腔发生病理变化时，这种78K蛋白具有出现的趋势（84％）。从临床检查的结果来看，没有78K蛋白的病例具有慢性或固定症状的趋势。通过统计分析，78K蛋白的外观与磁盘畸形的发生率之间的加冕系数为0.58。该结果表明，磁盘畸形可能与78K蛋白的外观有一定的关系。在病理滑液中，仅通过分析乙酸纤维素乙酸酯电池电泳，糖胺聚糖（GAG）仅由透明质酸（HA）组成。作为插科打链，人类TMJ磁盘主要包含硫酸软骨素，而在其他硫酸软骨蛋白中，硫酸盐和HA，但对比，但对比，猪TMJ磁盘主要是硫酸皮肤硫酸盐，还有一些硫酸软骨蛋白硫酸硫酸盐和HA。