The Effects of TGF-Beta on the Tumor Proliferation and Bone Resorption by Human Squamous Cell Carcinoma (S. C. C.)

TGF-β对人鳞状细胞癌(S.C.C.)肿瘤增殖和骨吸收的影响

基本信息

  • 批准号:
    63480439
  • 负责人:
  • 金额:
    $ 4.54万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1988
  • 资助国家:
    日本
  • 起止时间:
    1988 至 1990
  • 项目状态:
    已结题

项目摘要

The effects of transforming growth Factor-beta (TGF-beta) on three human oral squamous cell carcinoma cell lines, HSC-2, 3, and HSC-4, were investigated. Although these cell lines were equally sensitive to epidermal growth factor, responses to TGF-beta were variable. Dose-dependent inhibition of cell growth and (^3H)-thymidine incorporation of HSC-4 was observed by addition of TGF-beta, whereas growth inhibitory effects on HSC-2 and HSC-3 were marginal. Also the bone resorptions at nude mice calvaria by HSC-2, 3, and HSC-4, were investigated. HSC-2 and HSC-3 were able to cause bone resorption of the nude mice calvaria, whereas HSC-4 was not. Scatchard analysis of the binding of TGF-beta suggested that all squamous cell carcinoma cell lines have similar binding properties, which two classes of binding sites for TGF-beta. Affinity labeling of ^<125>I-TGF-beta to cell surface receptors revealed the two major affinity cross-linked bands with M_rs of 65 kDa (typeI) and 280 kDa (type III). A concomitant loss of 85 kDa band (type II) was observed in all squamous cell carcinoma cell lines examined. The numbers of type I receptors of these cell lines are variable : That is type I receptors of HSC-4 are comparably more abundant than those of HSC-2,3. These results indicate that bone resorption by S.C.C. cell lines is related to TGF-beta's effect on cell growth of the cell lines and that these effects may be mediated by type I receptors.
研究了转化生长因子-β(TGF-β)对三种人口腔鳞状细胞癌细胞系HSC-2、3和HSC-4的作用。虽然这些细胞系对表皮生长因子同样敏感,但对TGF-β的反应是可变的。通过加入TGF-β观察到细胞生长和HSC-4的(^3H)-胸苷掺入的剂量依赖性抑制,而对HSC-2和HSC-3的生长抑制作用是轻微的。同时观察了HSC-2、3和HSC-4对裸鼠颅骨的骨吸收作用。HSC-2和HSC-3能够引起裸鼠颅骨的骨吸收,而HSC-4不能。对TGF-β结合的Scatchard分析表明,所有鳞状细胞癌细胞系具有相似的结合特性,其中两类结合位点为TGF-β。β<125>-TGF-β与细胞表面受体的亲和标记揭示了两个主要的亲和交联带,M_r为65 kDa(I型)和280 kDa(III型)。在所有检查的鳞状细胞癌细胞系中观察到85 kDa条带(II型)的伴随损失。这些细胞系的I型受体的数量是可变的:也就是说,HSC-4的I型受体比HSC-2的I型受体丰富得多,3。这些结果表明,S.C.C.细胞系的生长与TGF-β对细胞系的细胞生长的作用有关,并且这些作用可能由I型受体介导。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hidenori Ichijo: "Purification and characterization of recombinant human TGF-β1" European Journal of Biochemistry. (1990)
Hidenori Ichijo:“重组人 TGF-β1 的纯化和表征”《欧洲生物化学杂志》(1990 年)。
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    0
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Fumio MOMOSE: "Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas." J Oral Pthol Med,. 18,. 391-395, (1990)
Fumio MOMOSE:“在来自人类口腔鳞状细胞癌的裸鼠中选择具有不同转移潜能的变异亚系。”
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    0
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一條 秀憲: "TGFーβ" MEDICO. 21. 17-19 (1990)
一条英德:“TGF-β”MEDICO。21. 17-19 (1990)
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    0
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Shinichiro Oida: "Induction of fibronectin in human squamous cell carcinomas by osteoinductive factor from bovine tooth" Biochemical Biophysical Research Communication. (1990)
Shinichiro Oida:“通过牛牙的骨诱导因子诱导人鳞状细胞癌中的纤连蛋白”生化生物物理研究通讯。
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Fumio Momose: "Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomos." Journal of Oral Pathology and Medicine. 18. 391-395 (1990)
Fumio Momose:“在来自人类口腔鳞状细胞癌的裸鼠中选择具有不同转移潜能的变异亚系。”
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    0
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SHIODA Shigetoshi其他文献

SHIODA Shigetoshi的其他文献

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{{ truncateString('SHIODA Shigetoshi', 18)}}的其他基金

Studies on Application of Shape Memory Alloy Plate for Internal Fixation of Jaws
形状记忆合金板在颌骨内固定中的应用研究
  • 批准号:
    63870079
  • 财政年份:
    1988
  • 资助金额:
    $ 4.54万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B).

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