Adaptations of chloroplast protein transport mechanisms during land plant evolution

陆地植物进化过程中叶绿体蛋白运输机制的适应

• 批准号: 440370941
• 负责人: Professorin Dr. Danja Schünemann
• 金额: --
• 依托单位: Arbeitsgruppe Molekularbiologie pflanzlicher Organellen
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/440370941?language=en
• 关键词: Adaptations; chloroplast; protein; transport; mechanisms

Thylakoid membrane biogenesis requires the coordinated transport of nucleus- and plastid-encoded membrane proteins. The chloroplast signal recognition particle (SRP) system plays an important role in the post- and cotranslational transport of a subset of these proteins. Previous research has shown that these transport mechanisms underwent striking molecular adaptations with the evolution of streptophyte algae and land plants. In the proposed project we aim to get further insight into the molecular changes of the SRP system during land plant evolution and to reveal the underlying evolutionary driving forces. We will particularly analyze (i) the function of the cpSRP proteins (cpSRP43, cpSRP54) and the SRP RNA, (ii) the regulation of SRP-dependent transport and (iii) the docking mechanism of ribosomes to the Alb3 insertase in the moss Physcomitrium patens and the liverwort Marchantia polymorpha. To this end, we will combine genetic, cell biological, biochemical, and structural approaches.

类囊体膜的生物发生需要核和叶绿体编码的膜蛋白的协调运输。叶绿体信号识别颗粒(SRP)系统在这些蛋白质的翻译后和共翻译后转运中起着重要作用。以往的研究表明，随着链藻和陆地植物的进化，这些运输机制经历了显着的分子适应。在拟议的项目中，我们旨在进一步深入了解SRP系统在陆地植物进化过程中的分子变化，并揭示潜在的进化驱动力。我们将详细分析(I)cpSRP蛋白(cpSRP43，cpSRP54)和SRP RNA的功能，(Ii)依赖SRP的运输的调节和(Iii)核糖体与多态苔藓和地钱草的Alb3插入酶的对接机制。为此，我们将结合遗传学、细胞生物学、生化和结构学方法。