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The role of VLA proteins and fibronectin in the activation of synovial cells from rheumatoid arthritis.

VLA 蛋白和纤连蛋白在类风湿性关节炎滑膜细胞激活中的作用。

基本信息

批准号：
03670333
负责人：
MATSUYAMA Takami
金额：
$ 1.34万
依托单位：
Kagoshima University (1992-1993)Saitama Medical University (1991)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Title : The role of VLA proteins and fibronectin in the activation of synovial cells from rheumatoid arthritis.In this study, our aim was to clarify whether the interaction of the very late antigen (VLA) on synovial cells from rheumatoid arthritis patients and that ligand, fibronectin is involvd in the pathogenesis of rheumatoid arthritis synovitis. First, we examined the expression of VLA proteins (VLA2, 3, 4, 5) on synovial tissues. VLA3, 4 and 5 proteins were expressed on the synovial lining and sublining cells. Lymphocytes mainly expressed VLA-4. Endothelial cells expressed VLA2, 3 and 5 proteins.When we compared the expression of VLA-4 protein among peripheral blood T cells, synovial T cells and synovial fluid T cells, we found the increased expression of VLA-4 on synovial fluid T cells, suggesting the existence of VLA-4 inducing substance in synovial fluid. Next, we examined whether fibronectin and that fragments affect the proliferation of synovial cells. Although they did not so, they induced the shape change of synovial A cells. When we detected the phosphorylated proteins in synovial cells after the stimulation of fibronectin, we did not get the constant pattern. Recently it was reported that the vascular cell adhesion molecule-1 (VCAM-1) was the other ligand of VLA-4 protein. Thereby, we examined the expression of VCAM-1 in the synovial tissues from rheumatoid arthritis patients. That expression was shown on the synovial lining and sublining cells. Since most of these cells express the VLA-4 protein, the interaction of these cells might be attributed to the binding of VLA-4 and VCAM-1 molecules.

题目：VLA蛋白和纤维连接蛋白在类风湿关节炎滑膜细胞活化中的作用。在这项研究中，我们的目的是阐明类风湿关节炎患者滑膜细胞上的极迟抗原（VLA）与配体纤维连接蛋白的相互作用是否参与类风湿关节炎滑膜炎的发病机制。首先，我们检测了VLA蛋白（vla2,3,4,5）在滑膜组织中的表达。VLA3、vla4和vla5蛋白在滑膜衬里和亚衬里细胞上表达。淋巴细胞主要表达vla4。内皮细胞表达VLA2、3和5蛋白。当我们比较外周血T细胞、滑膜T细胞和滑膜液T细胞中vla4蛋白的表达时，我们发现vla4在滑膜液T细胞中的表达增加，提示滑膜液中存在vla4诱导物质。接下来，我们研究了纤连蛋白及其片段是否影响滑膜细胞的增殖。虽然它们没有这样做，但它们诱导了滑膜A细胞的形状改变。当我们检测纤维连接蛋白刺激后滑膜细胞的磷酸化蛋白时，我们没有得到恒定的模式。近年来有报道称血管细胞粘附分子-1 （VCAM-1）是VLA-4蛋白的另一个配体。因此，我们检测了VCAM-1在类风湿关节炎患者滑膜组织中的表达。这种表达出现在滑膜衬里和下层细胞上。由于这些细胞大多表达vca -4蛋白，这些细胞的相互作用可能归因于vca -4和VCAM-1分子的结合。