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Basic study and clinical application of genetic diagnosis for lung cancer using polymerase chain reaction (PCR).

聚合酶链反应（PCR）肺癌基因诊断的基础研究及临床应用。

基本信息

批准号：
03807088
负责人：
KATO Harubumi
金额：
$ 1.15万
依托单位：
Tokyo Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807088/
关键词：
p53 PCR lung cancer p53 遺伝子診断 SSCP

项目摘要

Recently it has been reported that the tumor suppressor gene p53 is frequently mutated in primary lung cancer and various other types of cancer. So we examined the p53 mutation pattern in lung cancer by the PCR-SSCP(polymerase Chain reaction-single strand conformation polymorphism), a simple and sensitive method for detection of DNA mutation. And we analyzed its relationship with clinicopathological features. Tumor specimens were obtained from primary lung cancer of 45 patients, who were treated surgically at the Tokyo Medical College Hospital, Tokyo. Because 98% of p53 gene mutation in different cancers have been found in exon 5-8, we focused our study on these exons. The oligonucleotide primers for amplification of exons 5-8 were designed based on the published sequence. Fifteen of the 45 (33%) lung cancer cases showed abnormally shifted bands on PCR-SSCP analysis of the p53. The percentage of p53 mutation-positive cases varied to the histological type. In adenocarcinoma the incidence of cases detected p53 mutation showed a tendency to increase with advancing pathological stage. This result indicate that mutation of the p53 gene plays an important role in the development of primary lung cancer and its a potential prognostic factor in adenocarcinoma of the lung. We consider that a careful follow-up is necessary for case detected mutation of p53 gene.

最近有报道称，肿瘤抑制基因p53在原发性肺癌和其他各种类型的癌症中经常发生突变。因此，我们采用PCR-SSCP（polymerase Chain reaction-single - strand构象多态性，PCR-SSCP）这一简便、灵敏的DNA突变检测方法来检测肺癌中p53的突变模式。并分析其与临床病理特征的关系。肿瘤标本取自45例在东京医科大学医院接受手术治疗的原发性肺癌患者。由于在不同的癌症中，98%的p53基因突变发生在5-8外显子上，所以我们将研究重点放在了这些外显子上。根据已公布的序列设计扩增5-8外显子的寡核苷酸引物。在45例肺癌病例中，有15例（33%）在PCR-SSCP分析中显示p53条带异常移位。p53突变阳性病例的百分比因组织学类型而异。在腺癌中，p53突变的发生率随病理分期的进展而增加。提示p53基因突变在原发性肺癌的发生发展中起重要作用，是影响肺腺癌预后的潜在因素。我们认为对p53基因突变的病例进行仔细的随访是必要的。