SENTINAL LYMPH NODE MAPPING IN NON-SMALL CELL LUNG CANCER

本研究的目的：光敏剂荧光光诊断是早期癌症诊断的一种有前景的方法。本研究旨在评价荧光成像方法检测小鼠足垫肿瘤前哨淋巴结的可行性。材料和方法：用全彩色荧光成像系统检测含或不含Meth-A移植瘤的Bulb/C小鼠足垫的延髓淋巴结(PLN)(小鼠的SLN)。在麻醉的小鼠模型中，我们解剖了一条后肢，以显示肘结节和传入淋巴管。每种光敏剂都被注射到爪背皮下。采用全彩成像增强器的荧光诊断系统，在注射后60min获得淋巴结的荧光图像。每次注射光敏剂后10min，取小鼠窝结节，测量每个结节的荧光强度，并进行组织病理学检查。比较了三种不同的光敏剂：邻菲咯啉、N-天冬氨酸胆红素e6(Npe6)、ATX-S10。结果：观察了每种光敏剂的淋巴流动，并利用荧光图像检测到了小鼠窝结节的荧光。各光敏剂在注射后1min内观察到各光敏剂在小鼠淋巴管、淋巴管、淋巴管内的花期，并可在1小时内分辨出小鼠窝内点头。无论肿瘤有无转移，均可检测到窝窝结节的荧光。Npe6和ATX-S10的荧光强度与Photofrin大致相同且更强。结论：荧光诊断系统是前哨、结点标测的实用方法之一。荧光诊断是一种可行、安全的诊断方法。预计荧光诊断将对提高前哨淋巴结的检测水平有很大帮助。

Aim of this study : Fluorescence photodiagnosis with phosensitizers is one of the promising methods for diagnosis of early stage cancers. This study was designed to evaluate the fluorescence imaging method is feasible for detection of sentinel node of mouse footpad tumor.Material and methods : The popliteal lymph node (PLN)(the SLN in the mouse) of the footpad of Bulb/C mice, with or without Meth-A transplanted tumor, was detected using full-color fluorescence imaging system. In an anesthetized mouse model we dissected a hind limb to display the popliteal node and afferent lymphatic. Each photosensitizer was injected subdermally in the dorsum of the paw. Fluorescence images of lymph node were obtained for 60 minutes post injection using Fluorescence diagnosis system with full-color imaging intensifier. The popliteal nods were resected from mice ten minutes after each photosensitizer injection and the fluorescence intensity of the each node was measured and examined histopothologically. Three different photosensitizers, Phorofrin, N-aspertyl cholorin e6 (Npe6), ATX-S10 were compared.Results : The lymphatic flows of each photosensitizers were observed and the popliteal nods emitting fluorescence were detected in mice using Fluorescence images. The florescence of each photosensitizers in lymphatic, flows and a popliteal nod in mice were observed within 1mimute after injection and could be distinguish about 1 hour. The fluorescence form a popliteal nod could be detected either tumor was metastasized or not. The fluorescence magnitudes of Npe6 and ATX-S10 were about same and stronger than that of Photofrin.Conclusion : This result suggests that with fluorescence diagnosis system is one of utility method of Sentinel,node mapping. The Fluorescence diagnosis is a feasible and safe. It is expected that the Fluorescence diagnosis will go a long way toward improvement of detecting sentinel lymph node.