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SENTINAL LYMPH NODE MAPPING IN NON-SMALL CELL LUNG CANCER

非小细胞肺癌的前哨淋巴结定位

基本信息

批准号：
14571282
负责人：
KATO Harubumi
金额：
$ 1.6万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Aim of this study : Fluorescence photodiagnosis with phosensitizers is one of the promising methods for diagnosis of early stage cancers. This study was designed to evaluate the fluorescence imaging method is feasible for detection of sentinel node of mouse footpad tumor.Material and methods : The popliteal lymph node (PLN)(the SLN in the mouse) of the footpad of Bulb/C mice, with or without Meth-A transplanted tumor, was detected using full-color fluorescence imaging system. In an anesthetized mouse model we dissected a hind limb to display the popliteal node and afferent lymphatic. Each photosensitizer was injected subdermally in the dorsum of the paw. Fluorescence images of lymph node were obtained for 60 minutes post injection using Fluorescence diagnosis system with full-color imaging intensifier. The popliteal nods were resected from mice ten minutes after each photosensitizer injection and the fluorescence intensity of the each node was measured and examined histopothologically. Three different photosensitizers, Phorofrin, N-aspertyl cholorin e6 (Npe6), ATX-S10 were compared.Results : The lymphatic flows of each photosensitizers were observed and the popliteal nods emitting fluorescence were detected in mice using Fluorescence images. The florescence of each photosensitizers in lymphatic, flows and a popliteal nod in mice were observed within 1mimute after injection and could be distinguish about 1 hour. The fluorescence form a popliteal nod could be detected either tumor was metastasized or not. The fluorescence magnitudes of Npe6 and ATX-S10 were about same and stronger than that of Photofrin.Conclusion : This result suggests that with fluorescence diagnosis system is one of utility method of Sentinel,node mapping. The Fluorescence diagnosis is a feasible and safe. It is expected that the Fluorescence diagnosis will go a long way toward improvement of detecting sentinel lymph node.

目的：利用光敏剂进行荧光光诊断是早期癌症诊断的一种很有前途的方法。本研究旨在评价荧光成像方法检测小鼠足垫肿瘤前哨淋巴结的可行性。材料与方法：采用全彩荧光成像系统检测有或无甲氧a移植瘤的Bulb/C小鼠足垫腘窝淋巴结（PLN）（小鼠的SLN）。在麻醉小鼠模型中，我们解剖后肢以显示腘窝淋巴结和传入淋巴。每种光敏剂在爪背皮下注射。使用荧光诊断系统及全彩成像增强器获得注射后60分钟淋巴结的荧光图像。每次注射光敏剂10分钟后，取小鼠腘窝淋巴结，测定各淋巴结的荧光强度并进行组织病理学检查。比较了三种不同的光敏剂：磷磷、n -曲霉酰氯e6 （Npe6）、ATX-S10。结果：观察了各光敏剂的淋巴流动，荧光图像检测了小鼠腘窝淋巴结的荧光。各光敏剂在注射后1min内观察到小鼠淋巴、血流和腘窝淋巴结的荧光，约1 h可区分。腘窝淋巴结的荧光可以检测肿瘤是否转移。Npe6和ATX-S10的荧光强度基本相同，且比Photofrin更强。结论：荧光诊断系统是前哨、节点定位的实用方法之一。荧光诊断是一种可行、安全的方法。预计荧光诊断将对前哨淋巴结的检测有很大的帮助。