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Classification of Dentin Caries based on pH and Acid Profile of Carious Dentin and its Clinical Application

基于龋齿牙本质pH值和酸度的牙本质龋齿分类及其临床应用

基本信息

批准号：
03807122
负责人：
HOJO Sachiko
金额：
$ 1.22万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807122/
关键词：
Caries Dentin pH Acid HPLC 象牙質ウ蝕ウ蝕象牙質

项目摘要

In this study, we analyzed organic acids in carious dentin and dental plaque by HPLC. In carious dentin, lactate (mean: 30.7% of total acids) and acetate (mean: 36.7%) were the major acids as we reported previously. However, a high amount of formate (mean: 13.4%) and succinate (mean: 11.2%) were detected in the arrested type carious dentin, although only a little amount of formate and succinate were detected in the active type carious dentin. Formate and succinate in carious dentin were confirmed by Carboxylic Acid Analyzer and enzymatic assay. Existence of high amount of formate in carious dentin implies that bacteria having pyruvate formate-lyase are living in the arrested caries lesions and that the conditions in the lesions are strictly anaerobic. Detection of plenty of succinate in carious dentin indicates that succinate-producing bacteria, such as Actinomyces, Bacteroides, Propionibacterium, and Arachnia, are harboring in the arrested caries lesions. Furthermore, we compared organic acids among carious dentin, dental plaque covered on the caries lesion, and dental plaque on the sound teeth in the same mouth. Both active carious dentin and dental plaque on the caries lesion contained high amount of lactate over after being starved for long time, whereas dental plaque on the sound tooth surface had little lactate without sugar-intake. This difference implies that dentin caries establish a stable environment in which acids were produced are not easily washed away by saliva.

在本研究中，我们通过 HPLC 分析了龋齿牙本质和牙菌斑中的有机酸。在龋齿牙本质中，乳酸（平均：总酸的 30.7%）和乙酸盐（平均：36.7%）是我们之前报道的主要酸。然而，在停滞型龋齿牙本质中检测到大量的甲酸（平均：13.4%）和琥珀酸（平均：11.2%），而在活性型龋齿牙本质中仅检测到少量的甲酸和琥珀酸。通过羧酸分析仪和酶法测定龋齿牙本质中的甲酸盐和琥珀酸盐。龋齿牙本质中存在大量甲酸意味着具有丙酮酸甲酸裂解酶的细菌生活在被抑制的龋损病变中并且病变中的条件是严格厌氧的。在龋齿牙本质中检测到大量琥珀酸表明，在被抑制的龋病病变中藏有产琥珀酸的细菌，如放线菌属、拟杆菌属、丙酸杆菌属和蜘蛛杆菌属。此外，我们还比较了同一口中龋齿牙本质、覆盖在龋齿病变上的牙菌斑以及健康牙齿上的牙菌斑中的有机酸。长期饥饿后，龋病灶上活跃的龋齿牙本质和牙菌斑都含有大量的乳酸，而健康牙表面的牙菌斑在不摄入糖分的情况下几乎没有乳酸。这种差异意味着牙本质龋齿建立了一个稳定的环境，其中产生的酸不容易被唾液冲走。