Enzyme histochemical research of mononuclear cells and multinuclear-giant cells on the pressure sides in the periodontium incidental to experimental tooth movement

实验性牙齿移动中牙周膜压力侧单核细胞和多核巨细胞的酶组织化学研究

基本信息

批准号：
03807125
负责人：
HIRASHITA Ayao
金额：
$ 1.22万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

The purpose of this study was to elucidate the relationship between monocyte-macrophages and osteoclasts on the pressure sides in the periodontium incidental to experimental tooth movement. Enzymes of non-specific esterase (with or without NaF) were used for the identification of monocytes and macrophages, specific esterase was used for neutrophils and mast cells, tartrate-resistant acid phosphatase was used for preosteoclasts and mature osteoclasts.Results : 1. Monocytes and macrophages were observed mainly near blood vessels, but most of cells were macrophages. 2. Many osteoclasts and a few preosteoclasts were seen on the surface of the alveolar bone and near the hyalinized tissues. 3. Neutrophils were seen only in the experiments of 1 and 12 hours'durations. 4. Mast cells were not observed in any of the experimental durations. 5. In bone marrow, many preosteoclasts and osteoclasts were observed. 6. The relationship between the appearances of macrophages and osteoclasts could not be correlated by an analysis of variance of the rations in the number of these cells after each periods.These results suggest that there may be a fundamental biological difference between monocyte-macrophages and osteoclasts, and that some osteoclasts formed by fusion of preosteoclasts in bone marrow may migrate in the periodontium through the bone channel.Now, experiments are in progress to study the effect of tooth movement on the changes in serum bone gla protein(BGP) which osteoblasts synthesize / secrete and osteoclasts release from the bone matrix by resorption.A part of these results suggests that the decrease (days 3-5) and the increase (days 7-21) of serum BGP contents incidental to tooth movement may reflect bone turnover in the periodontium.Further immunocytochemical study will be necessary for revealing of the intimate relationship between osteoblasts and osteoclasts incidental to tooth movement.

这项研究的目的是阐明牙周偶发到实验牙齿运动的压力侧的单核细胞巨噬细胞与破骨细胞之间的关系。使用非特异性酯酶（有或不带有NAF）的酶来鉴定单核细胞和巨噬细胞，特异性酯酶用于中性粒细胞和肥大细胞，将tartrate耐酸酸性磷酸酶用于骨骼前的细胞前和大多数细胞。巨噬细胞。 2。在肺泡骨的表面和透明组织附近看到许多破骨细胞和一些前细胞。 3。仅在1和12小时的实验中看到中性粒细胞。 4。在任何实验持续时间内都没有观察到肥大细胞。 5。在骨髓中，观察到许多前细胞和破骨细胞。 6. The relationship between the appearances of macrophages and osteoclasts could not be correlated by an analysis of variance of the rations in the number of these cells after each periods.These results suggest that there may be a fundamental biological difference between monocyte-macrophages and osteoclasts, and that some osteoclasts formed by fusion of preosteoclasts in bone marrow may migrate in the periodontium through the骨通道。现在，正在进行实验，以研究牙齿运动对成骨细胞合成 /分泌和破骨细胞的骨基质的骨基质的骨基质的变化（BGP）的影响。通过吸收骨基质从骨基质中释放。牙周牙周化学研究对于揭示成骨细胞与牙齿运动偶然的成骨细胞之间的亲密关系是必要的。