Study of coupling of the tension side during the first stage of tooth movement

牙齿移动第一阶段张力侧耦合研究

基本信息

批准号：
12672022
负责人：
HIRASHITA Ayao
金额：
$ 2.56万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672022/
关键词：
osteoclasts tension side pressine side sinus structure coupling phenomenon TRAP-positive cell bone marrow rear resorption 牽引側歯根膜骨表面圧迫側骨膜骨表面連続横断切片

项目摘要

The purpose of this study was to elucidate the actual number of osteoclasts on the tension side incidental to the first stage of experimental tooth movement. The upper first molars of adult male rats were moved by a force of about 10 g for 1,2,3,4, and 5 days, then stained with toluidine blue and tartrate-resistant acid phosphatase (TRAP). We examined three vertical (every 100 μm) and four horizontal regions(from the periodontium to the periosteum), and counted the numbers of active(H-cell) and inactive (F-cell) osteoclasts in these areas under a light microscope. The following results were obtained ; (1). Most of the osteoclasts were H-cells and only a few F-cells. (2) The number of osteoclasts on the bone surface and half of the alveolus at the periodontal site peaked on day 4. (3). An increase in the number of osteoclasts on the bone surface of the periosteum and half of the alveolus at the periosteal site (rear resorption) was seen on days 4〜5. (4) There were many mononuclear cells with positive reaction of TRAP in the control bone marrow of day 4, but in experiments of day 4, multi-nuclear cells(osteoclasts) with TRAP reaction were observed. (5) In the deeper alveolar bone, many osteoclasts on the bone surface of periondontal sites in contrast to those of periosteal sites were observed already. These results suggest a necessary appearance of osteoclasts in the beginning (days 4〜5) of bone resorption and formation at the periodontal / periosteal sites on the tension area incidental to experimental tooth movement. Furthermore, these osteoclasts suggest to be recruited from the bone marrow in the experiments of day 4.

这项研究的目的是阐明在实验牙齿运动的第一阶段的张力侧的实际破骨细胞数量。成年雄性大鼠的上部磨牙通过约10 g的力移动1,2,3,4和5天，然后用甲苯胺蓝和耐锈蚀酸性酸性磷酸酶（TRAP）染色。我们检查了三个垂直（每100μm）和四个水平区域（从牙周到骨膜），并计算在光学显微镜下这些区域中的活动（H细胞）和非活动（F细胞）破骨细胞的数量。获得以下结果；（1）。大多数破骨细胞是H细胞，只有几个F细胞。（2）在第4天（3）的牙周位点上的骨表面上的破骨细胞数量和牙周位点的一半。在第4-5天，观察到骨膜骨表面上的破骨细胞数量增加（骨膜部位点）（后部分辨）的一半。（4）在第4天的对照骨髓中有许多单核细胞在对照骨髓中具有阳性反应，但是在第4天的实验中，观察到多核细胞（破骨细胞）与陷阱反应。（5）在较深的肺泡骨中，已经观察到与骨膜部位的骨骨表面上的许多破骨细胞。这些结果表明，在牙周 /骨膜位点的骨骼分辨率和形成的开始（第4-5天）的牙周 /骨膜位点的骨骼张力区域附带牙齿运动的牙周 /骨膜位点的形成时，破骨细胞的必要外观。此外，这些破骨细胞表明在第4天的实验中将从骨髓中募集。