Glutatlione Response of Hydra and Its Modulation by Autocline Mechanism.

水螅的谷胱甘肽反应及其自分泌机制的调节。

• 批准号: 03808034
• 负责人: HANAI Kazumitsu
• 金额: $ 0.96万
• 依托单位: Molecular Neurobiology Research Center, Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03808034/
• 关键词: Hydra; Growth Factor; Glutathione Chemoreception; Behavioral Response; Monoclonal Antibody; Glutathione Chemoreceptor; グルタチオン受容蛋白; モノクロ-ナル抗体; 核酸; 刺激細胞

The fresh water coelenterate, Hydra, responds to reduced glutathione to yield the feeding response. When Hydra japonica is cultured in a specific medium, the feeding response is the tentacle ball formation which is sensitively modulated by growth factors from mammalian animals. The response is composed of at least 5 components (R1-R5) on the basis of the modulation by growth factors. Monoclonal antibodies (mAb) which modulated the specific subset of the response were prepared. With use of two mAbs J245 and J5 among these mAbs, both of which depressed R5, the antigen was analyzed in detail to disclose molecular mechanism of the response. When these two mAbs were applied at the same time, the depression on R5 was the same as each mAb was applied separately within an experimental error, implying that these two mAbs has an effect on the same receptor responsible for R5. After labeling the antigen with a photoaffinity ligand of [35S]-glutathione, the labeled antigen was about 200 kDa on a HPLC gel permeation chromatography. When the J245 antigen was analyzed with J5 on the protein blotting, 180, 200 kDa components were common protein of these two mAbs. These results strongly suggest that these proteins, common antigen of J245 and J5, are one of the receptors mediating R5 response.

淡水腔肠动物九头蛇对还原型谷胱甘肽产生摄食反应。当日本水螅在特定培养基中培养时，摄食反应是触须球的形成，触须球的形成受哺乳动物生长因子的敏感调节。在生长因子调制的基础上，该响应由至少5个分量（R1-R5）组成。制备了单克隆抗体（mAb），可调节该反应的特定亚群。利用其中抑制R5的两种单克隆抗体J245和J5，对抗原进行了详细分析，揭示了应答的分子机制。当这两种单抗同时应用时，在实验误差范围内，对R5的抑制是相同的，因为每种单抗都是单独应用的，这意味着这两种单抗对负责R5的同一受体起作用。用[35S]-谷胱甘肽光亲和配体标记抗原后，在HPLC凝胶渗透层析上标记的抗原约为200 kDa。用J5对J245抗原进行蛋白印迹分析，发现这两种单克隆抗体的共同蛋白成分分别为180、200 kDa。这些结果强烈提示这些蛋白是介导R5应答的受体之一，它们是J245和J5的共同抗原。

项目成果

Kuriyama,K., Nakayasu,H.,Mizutani,H.,Hanai,K.,Kimura,H.: "Structure and function of GABA_β receptor in bovine cerebral cortex: analysis using the purified receptor and monoclonal antibody." Pharmacology Comm.2. 15-19 (1992)

Kuriyama, K.、Nakayasu, H.、Mizutani, H.、Hanai, K.、Kimura, H.：“牛大脑皮层 GABA_β 受体的结构和功能：使用纯化的受体和单克隆抗体进行分析。” 2. 15-19 (1992)

K.HANAI: "Serum levels and immunohistochemical brain localization in rats given diets deficient in L-lysine or protein." Phiysiology & Behavior. (1992)

K.HANAI：“饮食中缺乏 L-赖氨酸或蛋白质的大鼠的血清水平和免疫组织化学脑定位。”

Sasaki,K.,Oomura,Y.,Suzuki,K.,Hanai,K.,Yagi,H.: "Acidic fibroblast growth factor prevents death of hippocampal cal pyramidal cells following ischemia." Neurochem.Int.21. 397-402 (1992)

Sasaki,K.、Oomura,Y.、Suzuki,K.、Hanai,K.、Yagi,H.：“酸性成纤维细胞生长因子可防止缺血后海马锥体细胞死亡。”

Sasaki,K., Oomura,Y., Suzuki,K., Hanai,K., Yagi,H.: "Acidic fibroblast growth factor prevents death of hippocampal cal pyramidal cells following ischemia." Neurochem. Int. 21. 397-402 (1992)

Sasaki,K.、Oomura,Y.、Suzuki,K.、Hanai,K.、Yagi,H.：“酸性成纤维细胞生长因子可防止缺血后海马钙锥体细胞死亡。”

Torii.K.Hanai.K,Oosawa,K.,Funaba,M,Okiyama,A,Mori,M,Murata,T,Takahashi,M: "Serum levels and immunohistochemical brain localization in rats given diest deficient in L-lysine or protein." Phiysiology & Behavior. (1992)

Torii.K.Hanai.K，Oosawa，K.，Funaba，M，Okiyama，A，Mori，M，Murata，T，Takahashi，M：“给予缺乏 L-赖氨酸的大鼠的血清水平和免疫组织化学脑定位