Glutatlione Response of Hydra and Its Modulation by Autocline Mechanism.

淡水腔肠动物九头蛇对还原型谷胱甘肽做出反应以产生摄食反应。当九头蛇在特定的介质中培养时，摄食反应是触角球的形成，这是由哺乳动物的生长因子敏感地调节的。在生长因子调控的基础上，该响应至少由5个组分(R1-R5)组成。制备了调节特定反应亚群的单抗(MAb)。利用其中两株抑制R5的单抗J5和J45，对其抗原进行了详细的分析，以揭示其反应的分子机制。当这两种单抗同时应用时，对R5的抑制作用与各自单独应用时的实验误差相同，这意味着这两种单抗对负责R5的同一受体有作用。用[35S]-谷胱甘肽的光亲和配体标记抗原后，经高效液相色谱检测，标记的抗原大小约为200 kDa。用J5进行蛋白印迹分析，两株单抗的共同蛋白为180、200 kDa。这些结果有力地表明，这些蛋白是J245和J5的共同抗原，是介导R5反应的受体之一。

The fresh water coelenterate, Hydra, responds to reduced glutathione to yield the feeding response. When Hydra japonica is cultured in a specific medium, the feeding response is the tentacle ball formation which is sensitively modulated by growth factors from mammalian animals. The response is composed of at least 5 components (R1-R5) on the basis of the modulation by growth factors. Monoclonal antibodies (mAb) which modulated the specific subset of the response were prepared. With use of two mAbs J245 and J5 among these mAbs, both of which depressed R5, the antigen was analyzed in detail to disclose molecular mechanism of the response. When these two mAbs were applied at the same time, the depression on R5 was the same as each mAb was applied separately within an experimental error, implying that these two mAbs has an effect on the same receptor responsible for R5. After labeling the antigen with a photoaffinity ligand of [35S]-glutathione, the labeled antigen was about 200 kDa on a HPLC gel permeation chromatography. When the J245 antigen was analyzed with J5 on the protein blotting, 180, 200 kDa components were common protein of these two mAbs. These results strongly suggest that these proteins, common antigen of J245 and J5, are one of the receptors mediating R5 response.