Behavioral and Physiolosical Characteristics of the Animal Model of Mental Retardation (Fetal Alcohol Syndrome)

智力低下（胎儿酒精综合症）动物模型的行为和生理特征

• 批准号: 04610046
• 负责人: NAKAMURA Keisuke
• 金额: $ 0.9万
• 依托单位: Fukui University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04610046/
• 关键词: Fetal Alcohol Syndrome; Rat; Locomotor Activity; Circadian Activity Rhythm; Development of Circadian Rhythmicity; Re-entrainment of Circadian Rhythms; Passive Avoidance Learning Task; Rhythmic Activity of Hippocampal EEG

This study aimed to elucidate the effects of prenatal alcohol exposure on circadian activity rhythms, passive avoidance learning, and rhythmic activity of hippocampal EEG in the rat. Subjects were female albino rats of the Wistar-Imamichi strain and their offspring. Female rats of experimental group were administered 6% alcohol solution ad libitum as the sole fuluid source during 0-18 days of gestation, while after these treatment, mother rats and also their offspring were provided with water. Control rats were provided with water ad libitum throuthout the experiment. Activity test was performed with 8 pups in each group under the LD12 : 12 condition and then under the DD condition during 21-85 days after birth. Alcohol-exposed offspring were more active throughout the experiment than control offspring. Re-entrainment of circadian rhythms after 6 hours advanced or delayd phase-shifts of the LD12 : 12 illumination could be seen in all offspring in both groups, though alcohol-exposed offspring re-entrained more rapidly after both phase-shifts than control rats. Passive avoidance test was performed with 46 pups in each group, during 10 days from 21, 46, 70 days of age after birth respectively. Alcohol-exposed offspring required more trials for extinction than control offspring. There were no significant differences on activity and on emotionality during retention test traials between two groups, though the incidence of urination in the alcohol-exposed group decreased quickly compared with those in the control group. Hippocampal EEG were recorded monopolarly from 10 offspring in each group during voluntary movements, such as walking, locomotion, realing, head movement and sniffing, and during REM sleep. In the control group, appearances of hippocampal rhythmic slow activity were remarkable during voluntary movements, especially during walking, locomotion and rialing, and also during REM sleep. Alcohol-exposed offspring showed unclear rhythmic slow activity during volun

本研究旨在探讨产前酒精暴露对大鼠昼夜活动节律、被动回避学习和海马脑电节律活动的影响。受试者是Wistar-Imamichi品系的雌性白化病大鼠及其后代。实验组雌性大鼠在妊娠0-18 d内，以6%乙醇溶液作为唯一的氟源，自由采食，给母鼠及仔鼠饮水。对照组大鼠在实验期间自由饮水。在出生后21-85天内，每组8只幼鼠分别在LD 12：12条件下和DD条件下进行活动试验。在整个实验过程中，酒精暴露的后代比对照后代更活跃。在两组的所有后代中，在LD 12：12照明的6小时提前或延迟相移后，可以看到昼夜节律的再夹带，尽管酒精暴露的后代在两个相移后比对照组大鼠更快地再夹带。分别于生后21、46、70日龄进行被动回避试验，每组46只。暴露于酒精的后代比对照后代需要更多的灭绝试验。两组在保持试验中的活动性和情绪性无显著差异，但酒精暴露组排尿发生率较对照组迅速下降。每组各10只仔鼠，在自主运动（如行走、运动、站立、头部运动和嗅闻）和快速眼动睡眠期间，单次记录海马脑电图。对照组在自主运动时，尤其是在步行、运动和Rialing时，以及REM睡眠时，海马节律性慢活动出现明显。酒精暴露的后代表现出不明确的节奏缓慢的活动，

项目成果

Arai Hiroko and Nakamura Keisuke: "Relationship of Light-Dark Cycles and Photic-synchronization of Circadian Activity Rhytyms in Rats" Fukui Research of Special Education(in Japanese). (in press). 14 (1994)

新井博子和中村圭介：“大鼠昼夜节律活动节律的光暗周期和光同步的关系”福井特殊教育研究（日语）。

Nakamura Keisuke: "Congenital Anormaly in Offspring of Maternal Alcohol Consumption during Pregnancy (in Japanese)" The Japanese Journal of Special Education. 31-2. 57-63 (1993)

中村圭介：“怀孕期间母亲饮酒导致后代先天性异常（日语）”《日本特殊教育杂志》。

中村圭佐: "妊娠期アルコール摂取による先天性障害" 特殊教育学研究(日本特殊教育学会). 31. 57-63 (1993)

Keisa Nakamura：“怀孕期间饮酒引起的先天性疾病”特殊教育研究（日本特殊教育协会）31. 57-63（1993）。

荒井博子: "明暗周期がラットのサーカディアンリズムに与える影響" 福井大学 障害児教育研究. 14(印刷中). (1994)

Hiroko Arai：“明暗循环对大鼠昼夜节律的影响”福井大学残疾儿童教育研究14（出版中）。

荒井博子: "明暗周期がラットのサーカディアンリズムに与える影響" 福井大学障害児教育研究. 14(印刷中). (1994)

Hiroko Arai：“明暗循环对大鼠昼夜节律的影响”福井大学残疾儿童教育研究14（出版中）。