Studies on blue copper proteins and metal complexes as electron acceptors form a reduced enzyme

蓝铜蛋白和金属配合物作为电子受体形成还原酶的研究

• 批准号: 04640580
• 负责人: SUZUKI Shinnichiro
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640580/
• 关键词: blue copper protein; azurin; intermolecular electron-transfer; nitrite reductase; methylamine dehydrogenase; シュウドアズリン

The electron-transfer rates form Methylomonas J methylamine dehyrogenase to five blue copper proteins have been evaluated by cyclic voltammetry. The order of the electron-transfer rate constants from these copper proteins to methylamine dehydrogenase (E_<1/2>=ca.+100mV(NHE))) is as follows : azurin from Alcaligenes sp. (Az-As ; E_<1/2>=+270 mM(NHE)) > amicyanin from Methylobacterium extorquens AM1 (Am-Me ; +248 mV) > azurin-1 from Methylomonas J (Az-MJ1 ; +278 mV). Az-Az exhibited the higher activity bya factor of 7.5 as electron acceptors than Az-MJ2, but pseudoazurin from Methylomonas extorquens AM1 (pAz-Ac ; +260 mV) had hardly the activity. These findings are not due to the redox potentiols of blue copper proteins but the alignment of protein sequences. Espectially the hydrophobic phtch around hystidine imidazole guroup bound to Cu(II) in blue copper protein seems to be important to interact with methylamine dehydrogenase.

用循环伏安法测定了甲基单胞菌J甲胺脱氢酶对5种蓝铜蛋白的电子转移速率。从这些铜蛋白到甲胺脱氢酶的电子转移速率常数(E_&lt；1/2&gt；=约+100 mV(NHE))的顺序如下：产碱菌属的天青素。(AZ-AS；E_&lt；1/2&gt；=+270 mM(NHE))和GT；amilanin(Am-Me；+248 mV)和Gt；azurin-1(Az-MJ1；+278 mV)。Az-Az作为电子受体的活性比Az-MJ2高7.5倍，而来自甲基单胞菌AM1的假天青素(PAZ-Ac；+260 mV)几乎没有活性。这些发现不是由于蓝铜蛋白的氧化还原电势，而是蛋白质序列的比对。尤其是蓝铜蛋白中与铜(II)结合的咪唑基团周围的疏水分子似乎对甲胺脱氢酶的作用很重要。

项目成果

Takamitsu Kohzuma,Sohsuke Shidara,Shinnichiro Suzuki: "Direct Electrochemistry of Nitrite Reductase from Achromobacter cycloclastes IAM 1013." Bull.Chem.Soc.Jpn.67. 138-143 (1994)

Takamitsu Kohzuma、Sohsuke Shidara、Shinnichiro Suzuki：“无色杆菌环碎菌 IAM 1013 中亚硝酸盐还原酶的直接电化学。”

Shinnichiro Suzuki: "ESP of Copper Proteins" J.Act. Oxyg. Free Fad.3, (3). 341-349 (1992)

Shinnichiro Suzuki：“铜蛋白的 ESP”J.Act。

Takamitsu Kohzuma,Sohsuke Shidara,Kazuya Yamaguchi,Nobuhumi Nakamura,Deligeer,Shinnichiro Suzuki: "Direct Electrochemistry of Copper-Containing Nitrite Reductase from Achromobacter xylosoxidans NCIB 11015." Chem.Lett.1993. 2029-2032 (1993)

Takamitsu Kohzuma、Sohsuke Shidara、Kazuya Yamaguchi、Nobuhumi Nakamura、Deligeer、Shinnichiro Suzuki：“来自木糖氧化无色杆菌 NCIB 11015 的含铜亚硝酸盐还原酶的直接电化学。”

Shinnichiro Suzuki, Seiji Takase, Sohsuke Shidara, and Takamitsu Kohzuma: "Effect of Type II Copper in Enzyme Activity of Copper-containing Nitrite Reductase" J.Inorg. Chem.47, (1). 59 (1992)

Shinnichiro Suzuki、Seiji Takase、Sohsuke Shidara 和 Takamitsu Kohzuma：“II 型铜对含铜亚硝酸还原酶活性的影响”J.Inorg。

Takamitsu Kohzuma: "Direct Electrochemistry of Nitrite Reductase from Achromobacter cycloclastes IAM 1013." Bull.Chem.Soc.Jpn.67. 138-143 (1994)

Takamitsu Kohzuma：“来自无色杆菌环碎菌 IAM 1013 的亚硝酸盐还原酶的直接电化学。”