TYPE 1 COPPER COORDINATION IN THE ABSENCE OF SULFUR

不含硫时的 1 型铜配位

• 批准号: 8362235
• 负责人: KYLE M LANCASTER
• 金额: $ 0.27万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362235
• 关键词: Azurin; Copper; Electron Transport; Electronics; Funding; Grant; Kinetics; Ligation; National Center for Research Resources; Oxidation-Reduction; Principal Investigator; Process; Pseudomonas aeruginosa; Radiation; Research; Research Infrastructure; Resources; Site; Source; Sulfur; United States National Institutes of Health; absorption; cost; mutant; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have expressed and purified mutants of Pseudomonas aeruginosa azurins which possess reduction potentials and EPR spectra akin to type 1 (T1) or ?blue copper? sites despite lack of thiolate ligation. To date research has indicated the necessity of thiolates for T1 coordination. In concert with ongoing electronic absorption, EPR, and electron transfer kinetics studies, we wish to use XAS and EXAFS analysis to elucidate the origin of our peculiar EPR spectra as well as to assess site reorganization during oxidation/reduction processes.

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