Explanation of the diversity of photobionts in Cladia aggregate (Lichenes)

克拉迪亚聚集体（地衣）中光生物多样性的解释

• 批准号: 04640642
• 负责人: NAKANO Taketo
• 金额: $ 1.09万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640642/
• 关键词: Lichenes; Cladia aggregata; Diversity; Photobiont; Resynthesis; Tissue culture; Taxonomy

Photobionts were isolated from Cladia aggregata collected from some districts in Japan. From one podetium of Cladia aggregata collected from Kagoshima and Hiroshima, three species of photobionts were isolated. From podetia collected from other districts in Japan, one to two photobionts were isolated. They were Trebouxia erici, T.glomerata and T.pyriformis. As the results, the diversity of photobiont in Cladia aggregata was became clear at the first. The existence of three species of photobionts in only one podetium was newly found in the world. The distribution of photobionts in one podetium was uniform. By the tissue culture of Cladia aggregata, small thallus-like filaments were formed from some callus after one year on Malt-Yeast medium. Spores of mycobiont isolated from apotecia of Cladia aggregta were cultured and deposited in laboratory. They were used in resynthesis of Cladia aggregata in near future. The experiment of resynthesis is very important to make clear the diversity of photobionts in Cladia aggregata.

从日本部分地区采集的Cladia aggregata中分离出光合生物。从鹿儿岛和广岛采集的一株Cladia aggregata的柄上分离到3种光合生物。从日本其他地区收集的足类中，分离出一到两种光合生物。它们分别是梨形木蠹蛾（Trebouxia erici）、球状木蠹蛾（T.glomerata）和梨形木蠹蛾（T. pyriformis）。研究结果首次明确了Cladia aggregata光合生物的多样性。仅在一个柄中同时存在三种光合生物，这是世界上新发现的。光生菌在一个足中的分布是均匀的。通过对Cladia aggregata的组织培养，在麦芽-酵母培养基上培养一年后，部分愈伤组织形成了小的叶状体状细丝。对分离自Cladia aggregta的孢子进行了实验室培养和保藏。它们在不久的将来被用于Cladia aggregata的再合成。再合成实验对于弄清Cladia aggregata光合生物的多样性具有重要意义。