Diversity of the phycobiont in genus Stereocaulon (Lichenes)

1.对从日本和台湾采集的立枯草标本中分离到的培养沉淀物中的藻类进行了再调查。对1994~1995年采集的几种立体茎藻类标本中的藻类进行了分类研究。结果表明，仅从一种叶柄藻类中分离到2~3种不同种类的藻类，从而明确了立体藻类中藻类的物种多样性。2.利用野生空间菌对头藻中的光生生物进行了研究。由于次生藻类没有培养，因此在属水平上进行了鉴定。结果表明，在头藻中观察到的次生藻类被鉴定为蓝藻。但是，在立体茎属的一些物种中，只有一个头藻上有两到三个属的蓝藻。这一现象表明，物种多样性也存在次生藻类。3.利用外露链霉叶枝菌进行组织培养，并利用同种克隆真菌和藻酸进行重组。在这两个实验中，都诱导了愈伤组织和幼嫩的莲花体体。这些实验将在未来继续进行。

1.Phycobiont strains (prymary phycobiont) deposited by culture which were isolated from specimens of Stereocaulon collected in Japan and Taiwan were re-investigated. Phycobionts isolated specimens of some species of Stereocaulon collected from 1994 to 1995 also were studied taxonomically. As the result, two to three species differented on genus level as phycobionts were isolated from only one phyllocladium in some species of Stereocaulon and the species diversity of ohycobiont in Stereocaulon thallus was clarified.2.Photobions in cephalodia (secodary phycobiont) were investigated using wild spacimens. As secondary phycobionts did not cultured, they were identified in genus level. As the result, secondary phycobionts observed in cephalodia were identifield as cyanobacteria. But, in some species of Stereocaulon, two to three genera of cyanobacteria were observed in only one cephalodium. This phenomenon suggeste that the species diversity also exist secondary phycobionts.3.The tissue culture was made using phyllocladium of S.exutum and the reconstitution also was made using clonized fungi and algac of same species. In both experiments, callus and young liehen thalli were induced. This experiments are continued for the future.