Production of intergeneric hybrids of wasabi and horseradish by somatic cell fusion.

从离体植物获得的两个物种的成熟叶用于该实验。增加脉冲数可使细胞融合率提高17%，单对率提高8%。获得了有活力的小原生质体和胞质体。但未去核的原生质体仍保留下来，亚原生质体很少.进行了原生质体与微型原生质体、微型原生质体与胞质体、原生质体与胞质体的相互融合。通过原生质体与胞质的融合，将一个亲本细胞的细胞质顺利导入另一个亲本细胞。而电融合法细胞核内吸置换困难。培养含有替代细胞质的细胞的海藻酸凝胶珠。与液体培养相比，微珠培养更适合于本实验的目的。目前，我们正试图寻找诱导菌落形成的培养条件。

Mature leaves of both species obtained from the plants in vitro were used for this experiment. Percentage of formation of cell fusion and single pair was raised 17% and 8% by increase of pulse number, respectively.Removal of nucleus for asymmetrical hybridization was carried out by using Percoll. The vigorous miniprotoplast and cytoplast were obtained. However, the protoplasts which were not removed the nuclei remained, and subprotoplast was litte obtained. reciprocal fusion of protoplast and miniprotoplast, miniprotoplast and cytoplast, and protoplast and cytoplast was carried out. Cytoplasm of one parental cell was introduced smoothly another parental cell by the fusion of protoplast and cytoplast. In contrast, Introsuction and substitution of nuclear was difficult by electric cell fusion method. The beads of alginic acid gel which contained the cells substituted cytoplasm were cultured. The beads culture was suitable for this experimental purpose comparing with liquid culture. We are, presently, attempting search of culture conditions induced the formation of colony.