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Production of intergeneric hybrid between Eutrema wasabi and Armoracia rusticana, and sellection of favorable individuals.

山葵与黄山芥属间杂交种的生产及优良个体的选择。

基本信息

批准号：
02660029
负责人：
OHI Michio
金额：
$ 1.34万
依托单位：
Shinshu Univ.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660029/
关键词：
Intergeneric Crossing Eutrema wasabi Armoracia rusticana Cell Fusion Cvule Culture Ovary Culture 胚発育複二倍体電気融合

项目摘要

Pollen tubes in intergeneric crossing extended to ovule normally. The fertilized eggs were observed after 3 days pollinated in EPSILON. wasabiXALPHA. rusticana. But the rate of survival ovules decreased gradually after 10 days. Also fertilized eggs observed in ALPHA. rusticanaXEPSILON. wasabi, but disappeared after 25 days. The Hybrids of EPSILON. wasabiXALPHA. rusticana were germinated in ovule culture. The hybrids resembled closely EPSILON. wasabi in morphological characters. Results of chromosome investigation and isozyme analysis, all of germinated individuals were confirmed hybrids of EPSIOLN. wasabi and ALPHA. rusticana. In case of chromosome number, hybrid obtained 23 chromosomes as compared with 14 and 32 of each parent. For cell fusion of EPSILON. wasabi and ALPHA. rusticana, the protoplasts combined excellently on condition that VAC was 15-20V and INITIAL TIME was 25-35sec. . The best electric condition of fusion was 290-300V with VDC and 85-105us with PW.

属间杂交时花粉管正常伸长至胚珠。在EPSILON中授粉3 d后观察受精卵。wasabiXALPHA。rusticana。但10天后胚珠存活率逐渐下降。在ALPHA中也观察到受精卵。rusticanaXEPSILON。芥末，但25天后就消失了。EPSILON的混血儿。wasabiXALPHA。乡土植物在胚珠培养中萌发。这些杂交体与EPSILON非常相似。山葵的形态特征。染色体调查和同工酶分析结果表明，萌发个体均为EPSIOLN杂种。芥末和ALPHA。rusticana。就染色体数目而言，杂种获得23条染色体，而亲本各有14条和32条染色体。用于EPSILON细胞融合。芥末和ALPHA。在VAC为15 ~ 20v， INITIAL TIME为25 ~ 35sec的条件下，原生质体结合效果较好。熔合的最佳电条件为直流290 ~ 300v， PW 85 ~ 105us。