The establishment of the simple confirmation method of the flower-bud initiation in strawberry using the phenomenon of pectin accumulation in crown.

我们发现果胶在草莓花蕾形成过程中的积累现象。本研究旨在利用这一现象，建立一种简便的草莓花芽萌发鉴定方法。首先，我们尝试用从树冠中提取的果胶制作抗体。然而，从老鼠身上提取的抗体是低敏感性的，不实用。在此基础上，研究了直接测定果胶确定花芽起始的方法。试验选用4个品种。显微镜下确定花芽形成阶段后，用FAA固定冠部。从分化期到花蕾发育到雌蕊分化的早期阶段，果胶都积累到花冠上。果胶在FAA固定后第2天呈凝胶状沉积。分化期果胶积累量为5 ~ 500 mg /冠。将500 mg冠固定于FAA 10 ml中2天后，胶凝形式得到明确确认。在本研究中，尚不能建立抗原抗体反应确定花芽起始的方法。而用FAA法可以通过确定果胶在花芽形成过程中的积累来诊断花芽形成的开始。

We discovered the phenomenon that pectin accumulates in the strawberry crown according to the flower-bud initiation. Purpose of this research is to develop the easy confirmation method of the flower-bud initiation in strawberry by utilizing this phenomenon. First, we tried to make an antibody by using the pectin that extracted from the crown. However, the antibody from a mouse the was low sensitive, and not practical. Thereupon, we examined the method that flower-bud initiation is confirmed by measuring direct pectin. Four cultivars were used in the experiment. The crown was fixed with FAA after it was determined the stage of flower-bud initiation with a microscope. Until the development of flower-bud reaches to Early stage of pistil differentiation from Differentiated stage, the pectin was accumulated to the crown fairly plentifully in either cultivar. Pectin became a gel form deposit to the 2nd day after fixation with FAA.The accumulation quantity of the pectin in Differentiated stage is 5 mg to every 500 mg of crowns. Two days after 500 mg of the crowns were fixed to FAA 10 ml, the gel form pectin is confirmed clearly. It was not possible to establish the confirmation method of the flower-bud initiation by the antigen antibody reaction in this research. However, the start of flower-bud initiation is able to be diagnosed by confirming the accumulation of the pectin in the crown with flower-bud initiation by using FAA.