The establishment of the simple confirmation method of the flower-bud initiation in strawberry using the phenomenon of pectin accumulation in crown.

利用树冠果胶积累现象建立草莓花芽萌生的简便方法。

• 批准号: 10660022
• 负责人: OHI Michio
• 金额: $ 2.05万
• 依托单位: Faculty of Agriculture, Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660022/
• 关键词: Strawberry; Flower-bud initiation; Pectin; FAA fixation; gel; 抗原; 抗体; ペクチン質多糖類

We discovered the phenomenon that pectin accumulates in the strawberry crown according to the flower-bud initiation. Purpose of this research is to develop the easy confirmation method of the flower-bud initiation in strawberry by utilizing this phenomenon. First, we tried to make an antibody by using the pectin that extracted from the crown. However, the antibody from a mouse the was low sensitive, and not practical. Thereupon, we examined the method that flower-bud initiation is confirmed by measuring direct pectin. Four cultivars were used in the experiment. The crown was fixed with FAA after it was determined the stage of flower-bud initiation with a microscope. Until the development of flower-bud reaches to Early stage of pistil differentiation from Differentiated stage, the pectin was accumulated to the crown fairly plentifully in either cultivar. Pectin became a gel form deposit to the 2nd day after fixation with FAA.The accumulation quantity of the pectin in Differentiated stage is 5 mg to every 500 mg of crowns. Two days after 500 mg of the crowns were fixed to FAA 10 ml, the gel form pectin is confirmed clearly. It was not possible to establish the confirmation method of the flower-bud initiation by the antigen antibody reaction in this research. However, the start of flower-bud initiation is able to be diagnosed by confirming the accumulation of the pectin in the crown with flower-bud initiation by using FAA.

发现草莓花芽分化过程中，果胶在冠部积累的现象。本研究的目的是利用这一现象，建立草莓花芽分化的简易鉴定方法。首先，我们尝试用从皇冠中提取的果胶制备抗体。然而，来自小鼠的抗体是低灵敏度的，并且不实用。因此，我们研究了通过测量直接果胶来确认花芽起始的方法。试验选用4个品种。在显微镜下观察花芽分化阶段，用固定剂固定冠。从花芽分化期到雌蕊分化早期，果胶在两个品种的冠部都有较充分的积累。固定后第2天，果胶以凝胶状存款，在分化期每500 mg冠层中积累5 mg果胶。将500 mg冠状物固定到10 ml FAA上两天后，凝胶形式的果胶被清楚地证实。本研究尚无法建立以抗原抗体反应来确认花芽起始的方法。然而，花芽分化的开始能够通过使用FAA确认果胶在冠部中的积累和花芽分化来诊断。