The development of Fruit Tree cultivar identification using DNA fingerprinting

本研究是利用DNA指纹技术开发果树品种鉴定新应用的一次尝试。首先，研究人员检查了五种已知可以在人体中进行DNA指纹识别的探针与柑橘DNA杂交的能力。结果表明，牛M13噬菌体小卫星样结构域的克隆可用于柑橘品种的鉴定。其次，我们利用基于pcr的技术，利用小卫星序列检测DNA多态性。本研究以M13微卫星序列为基础合成了4条引物。结果表明，两引物组合可有效扩增多态片段。此外，由于部分PCR产物含有柑橘小卫星样序列，因此将其连接到质粒载体上用于DNA指纹识别。本研究为DNA序列信息贫乏的果树品种鉴定提供了良好的工具。

This study is an attempt to develop the new application of fruit tree cultivar identification using DNA fingerprinting.At first, five probes known to allow DNA fingerprinting in humans were checked for their ability to hybridize to Citrus DNA.The results showed that the clone of M13 phage minisatellite-like domain of bovine DNA was useful for the identification of Citrus cultivars.Secondly, we detected DNA polymorphisms with the PCR-based technique using minisatellite sequence. For this study, four primers were synthesized on the basis of M13 minisatellite sequence. The results showed that polymorphic fragments were amplified effectively by the combination of two primers.In addition, PCR products were ligated into the plasmid vector and used for DNA fingerprinting because some of them contained Citrus minisatellite-like sequences.We suggest that the strategy in this study is a good tool for cultivar identification of fruit tree which have poor information on DNA sequence.