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Phylogeny of east Asiatic bamboos analyzed by RFLPs of chloroplast DNA

利用叶绿体 DNA RFLP 分析东亚竹子的系统发育

基本信息

批准号：
06640895
负责人：
KOBAYASHI Mikio
金额：
$ 1.34万
依托单位：
Utsunomiya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Thirty-three bamboo genera, four close allies of Bambusoideae and Flagellaria indica (Flagellariaceae), as an outgroup, were analyzed for phylogenetic relationship by means of Southren hybridization-restriction fragment length polymorphism analysis of chloroplast DNA combined with the Wagner parsimony method. Fourteen restriction enzymes, all six-base cutters, and six fragments of rice cpDNA as probes (46% of total rice cpDNA) were used to produce in total 219 restriction site changes, of which 107 were detected as phylogenetically informative referring to the outgroup changes. A majority consensus tree obtained from equally parsimonous 36 best trees was found. The cladogram consisted of three major clades. The first was composed of all East Asiatic, cool temperate bamboos (13 genera). The second was composed of all tropical Asiatic bamboos (nine genera including Oxythenanthera abyssinica of Africa) and New World woody bamboos (four genera). These two were sister groups. Herbaceous bambusoid grasses of South America (five genera including Streptochaeta spicata) and a woody bamboo, Rhipidocladum ampliflorum, formed the third most basal clade. Three Oryzoideae spicies and Phaenosperma globosum fell into other clades which occurred between the former two and the latter one. These results suggest that world bamboos are polyphyletic and a phylogenetic origin of the world bamboos is more likely South America than Asia.

采用叶绿体DNA限制性片段长度多态性（RFLP）技术结合瓦格纳简约法，对竹亚科（Bambusoideae）的4个近缘属和鞭毛藻属（Flagellariindica）的33个竹属进行系统发育分析。用14种限制性内切酶、6个碱基切割器和6个水稻cpDNA片段作为探针（占水稻cpDNA总量的46%），共检测到219个限制性位点的变化，其中107个位点的变化与外群变化有关。从36棵最优树中得到一个多数一致的进化树，该进化树由3个主要分支组成。第一类是由所有的东亚，冷温带竹（13属）。第二类是所有热带亚洲竹类（包括非洲的Oxythenanthera abyssinica在内的9属）和新大陆木本竹类（4属）。这两个团体是姐妹团体。南美洲的草本类灌木草（包括穗状链毛藻（Streptochaeta spicata）在内的五个属）和一种木本竹子Rhipidocladum pastiflorum形成了第三个最基本的分支。3个Oryzoideae spaceae和Phaenosperma globosum分属于其他分支，它们位于前两者和后两者之间。这些结果表明，世界竹类是多系的，世界竹类的系统发育起源更可能是南美洲而不是亚洲。