权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Structure and function of gamma-polyglutamate gene in Bacillus subtilis(natto)

枯草芽孢杆菌（纳豆）γ-聚谷氨酸基因的结构与功能

基本信息

批准号：
04660123
负责人：
HARA Toshio
金额：
$ 1.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Natto, or non-salty fermented soybeans, is an aging product by the biological action of Bacillus subtilis(natto)grown on the steamed soybeans. Natto is covered with a viscous, sticky polymer that formed long, thin threads when touched. The main component of the viscous material is gamma-polyglutamate(gamma-PGA). The genetic character of high gamma-PGA productivity in B.subtilis(natto)was transferred to B.subtilis Marburg 168, which cannot produce gamma-PGA.Treatment of B.subtilis(natto)with acridine orange resulted designated pUH1 was detected in B.subtilis(natto)strain, but the stgderivatives were missing the 5.8-kb plasmid. The specific gene of natto plasmid pUH1, which is responsible for gamma-PGA production, has been cloned and the nucleotide sequence determined.There are many kinds of natto-like non-salty fermented soybean foods in Monsoon Asia. These natto-like fermented foods are considered to have streamed from the same cultural roots, which may be termed as "Natto Triangle". We might probably consider the center of this area as the roots of "Biotechnology" of our region. The natto plasmids were detected from the essential organisms for fermentation isolated from these natto-like fermented soybeans the natto plasmids, and by constructing the genealogical tree of these plasmids.Moreover, the unbalanced consumptions of natto in Japan would be discussed, including the origin of the Japanese.

纳豆，或称无盐发酵大豆，是由生长在蒸大豆上的枯草芽孢杆菌(纳豆)的生物作用而形成的陈化产品。纳豆表面覆盖着一种粘稠的聚合物，触摸时会形成细长的线条。粘性物质的主要成分是伽马-聚谷氨酸(伽马-PGA)。将枯草芽孢杆菌(B.subtilis(纳豆))高产γ-PGA的遗传特性转入不能产生伽马-PGA的枯草杆菌马尔堡168(B.subtilis Marburg 168)。用吖啶橙处理纳豆芽孢杆菌(B.subtilis(纳豆))后，在该菌株中检测到命名为pUH1的pUH1，但stg衍生物缺失了5.8kb的质粒。纳豆菌质粒pUH1负责产生γ-PGA的特异性基因已被克隆并测定了核苷酸序列。亚洲季风豆类发酵食品有多种纳豆类无盐发酵食品。这些类似纳豆的发酵食品被认为来自相同的文化根源，可以被称为“纳豆三角”。我们可能会认为这一地区的中心是我们地区“生物技术”的根源。从这些纳豆发酵大豆中分离出纳豆发酵必需微生物中的纳豆质粒，并通过构建这些质粒的谱系树，对纳豆在日本的不平衡消费进行了讨论，包括日本纳豆的起源。