Molecular and immmunological analyses of cytokines and γδT cells in intracellular infection of Salmonella
Molecular and immmunological analyses of cytokines and γδT cells in intracellular infection of Salmonella
批准号:
10470179
负责人:
HARA Toshio
金额:
$4.1万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999
中文摘要
摘要人类对沙门氏菌的宿主免疫应答知之甚少。本研究对沙门氏菌病患者外周血中Th1和Th-1细胞因子及T细胞反应进行了研究,发现沙门氏菌病患者急性期血清Th-1和Th-1细胞因子水平升高。此外,系统性沙门氏菌病患者血清IL-15、IL-18水平明显高于胃肠炎型患者。经系列检测,胃肠炎型患者血清干扰素-γ、IL-12、IL-15、IL-18水平较系统性胃肠炎患者更容易恢复正常,且干扰素-γ水平与IL-12、IL-18水平呈显著正相关。IL-15水平与IL-18水平呈显著正相关。体外沙门氏菌刺激后,沙门氏菌、卡介苗或大肠杆菌刺激均可产生IL-15和IL-18,而沙门氏菌刺激可优先产生IL-12和干扰素-γ。抗IL-12单抗和抗IL-18单抗均能显著抑制沙门氏菌诱导的干扰素-γ的产生,而抗IL-15单抗对其无明显抑制作用。系统性胃肠炎患者的HLADR+T细胞、CD69+T细胞、γδT细胞和HLADR+γδT细胞明显高于胃肠炎患者。HLA-DR+T细胞数与血清干扰素-γ、IL-15、IL-18水平呈正相关。HL A-DR+γδT细胞数与IL-15水平呈负相关。在系统性和胃肠炎中,分选的γδT细胞的干扰素-γ水平明显高于αβT细胞。综上所述,IL-12、IL-18和IL-15在复杂的相互作用下,似乎参与了整个T细胞的激活和γδT细胞的扩增,从而产生干扰素以保护人类免受沙门氏菌感染。
英文摘要
AbstractKnowledge of the host immune response against Salmonella is not sufficient in humans. In the present study, Th1- and Th-1 including cytokines and T cell response were investigated in salmonellosis.Serum Th-1 and Th-1including cytokine levels at acute phase in patients with salmonellosis were elevated. In addition, serum levels of IL-15 and IL-18 in patients with systematic form of salmonellosis were significantly higher than those in patients gastroenteritis form. By serial determination, serum IFN-γ ,IL-12, IL-15 and IL-18 levels in gastroenteritis form returned to normal levels easier than those in systematic form.IFN-γ level was significantly correlated with IL-12 and IL-18 levels. In addition, IL-15 level was significantly correlated with IL-18 level. Furthermore, IFN-γ , IL-15 and IL-18 levels were significantly correlated with duration with fever.Upon stimulation with Salmonella in vitro, IL-15 and IL-18 were produced by Salmonella, BCG or E. coli stimulation in both salmonellosis and controls, while IL-12 and IFN-γ were preferentially produced upon stimulation with Salmonella in Salmonellosis. A significant inhibition of Salmonella -induced IFN-γ production by anti-IL-12 mAb or anti-IL-18 mAb but not by anti-IL 15 mAb.HLA-DR+T cells, CD69+T cells, γδT cells and HLA-DR+γδT cells were significantly elevated in systematic form than in gastroenteritis from. HLA-DR+T cell-number tended to correlate with serum IFN-γ, IL-15 and IL-18 levels. HLA-DR+γδT cell-number was marginally associated with IL-15 levels. Sorted γδT cells contained significantly higher levels of IFN-γ mRNA than αβT cells both in systemic and gastroenteritis formsIn summary, IL-12, IL-18, and IL-15, with complex mutual interactions , appear to be involved in total T cell activation as well as γδT cell-expansion, resulting in IFN- production for the protection against Salmonella infection in humans.
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共 25 条
R&D of water-based liquid scintillator with Gd as a reactor anti-neutrino monitor
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批准号:23654089
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.41万
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财政年份:2011
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负责人:HARA Toshio
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依托单位:
A Study on the development of the System of Bookkeeping
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负责人:HARA Toshio
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依托单位:
The development of a liquid scintillator with Gd for nuclear reactor neutrino oscillation experiment DCHOOZ
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批准号:19540298
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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财政年份:2007
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负责人:HARA Toshio
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依托单位:
Research of a new liquid scintillator with Gd for the KASKA reactor neutrino oscillation experiment
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批准号:17540258
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.24万
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财政年份:2005
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负责人:HARA Toshio
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依托单位:
Structure and function of gamma-polyglutamate gene in Bacillus subtilis(natto)
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批准号:04660123
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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财政年份:1992
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负责人:HARA Toshio
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依托单位:
海外基金