Molecular and immmunological analyses of cytokines and γδT cells in intracellular infection of Salmonella

沙门氏菌细胞内感染中细胞因子和γδT细胞的分子和免疫学分析

基本信息

批准号：
10470179
负责人：
HARA Toshio
金额：
$ 4.1万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

AbstractKnowledge of the host immune response against Salmonella is not sufficient in humans. In the present study, Th1- and Th-1 including cytokines and T cell response were investigated in salmonellosis.Serum Th-1 and Th-1including cytokine levels at acute phase in patients with salmonellosis were elevated. In addition, serum levels of IL-15 and IL-18 in patients with systematic form of salmonellosis were significantly higher than those in patients gastroenteritis form. By serial determination, serum IFN-γ ,IL-12, IL-15 and IL-18 levels in gastroenteritis form returned to normal levels easier than those in systematic form.IFN-γ level was significantly correlated with IL-12 and IL-18 levels. In addition, IL-15 level was significantly correlated with IL-18 level. Furthermore, IFN-γ , IL-15 and IL-18 levels were significantly correlated with duration with fever.Upon stimulation with Salmonella in vitro, IL-15 and IL-18 were produced by Salmonella, BCG or E. coli stimulation in both salmonellosis and controls, while IL-12 and IFN-γ were preferentially produced upon stimulation with Salmonella in Salmonellosis. A significant inhibition of Salmonella -induced IFN-γ production by anti-IL-12 mAb or anti-IL-18 mAb but not by anti-IL 15 mAb.HLA-DR+T cells, CD69+T cells, γδT cells and HLA-DR+γδT cells were significantly elevated in systematic form than in gastroenteritis from. HLA-DR+T cell-number tended to correlate with serum IFN-γ, IL-15 and IL-18 levels. HLA-DR+γδT cell-number was marginally associated with IL-15 levels. Sorted γδT cells contained significantly higher levels of IFN-γ mRNA than αβT cells both in systemic and gastroenteritis formsIn summary, IL-12, IL-18, and IL-15, with complex mutual interactions , appear to be involved in total T cell activation as well as γδT cell-expansion, resulting in IFN- production for the protection against Salmonella infection in humans.

对沙门氏菌的宿主免疫响应的抽象知识在人类中还不够。在本研究中，在沙门氏菌病中研究了包括细胞因子和T细胞反应在内的Th1和Th-1。在沙门氏菌病患者的急性Th-1和Th-1中，包括急性相的细胞因子水平升高。此外，系统形式的沙门氏菌病患者的血清IL-15和IL-18水平明显高于患者胃肠炎的患者。通过串行确定，胃肠炎形式中的血清IFN-γ，IL-12，IL-15和IL-18水平比系统形式更容易恢复到正常水平。IFN-γ水平与IL-12和IL-18水平显着相关。另外，IL-15水平与IL-18水平显着相关。此外，IFN-γ，IL-15和IL-18水平与持续时间显着相关。通过沙门氏菌，BCG或大肠杆菌刺激在沙门氏菌和对照组中用沙门氏菌或大肠杆菌刺激产生了用沙门氏菌，IL-15和IL-18产生的，而IL-12和IL-12和IFN-γ的刺激性更高。抗IL-12 MAB或抗IL-18 MAB对沙门氏菌诱导的IFN-γ产生的显着抑制作用，而不是通过抗IL 15 mAb.HLA-DR+T细胞，CD69+T细胞，γΔT细胞，γδT细胞和HLA-DR+γδT细胞在系统形成中显着升高。 HLA-DR+T细胞数倾向于与血清IFN-γ，IL-15和IL-18水平相关。 HLA-DR+γδT细胞数与IL-15水平略有相关。在全身和胃肠炎中，与αβT细胞的水平明显更高，在全身和胃肠炎中，摘要，IL-12，IL-18和IL-15均具有复杂的相互作用，似乎与总T细胞活化以及γδT细胞相互作用有关，从而在IFN-Salmon中产生了γδT细胞的生产，从而参与了IFN-γδT细胞的生产。