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Free-Radical Scavenging Reation of beta-Carotene

β-胡萝卜素的自由基清除反应

基本信息

批准号：
04660137
负责人：
YAMAUCHI Ryo
金额：
$ 1.34万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660137/
关键词：
beta-Carotene Lipid peroxidation Antioxidant Free radical Peroxyl radical β‐カロテン

项目摘要

Carotenoids such as beta-carotene are considered to be effective inhibitors of lipid peroxidation. To know the mechanism of antioxidant activity of beta-carotene, it is important to analyze its oxidation products that may arise during its action as an antioxidant. In this research, the reaction products of beta-carotene with peroxyl radicals were isolated and characterized. beta-Carotene was reacted with an alkylperoxyl radical at 37ﾟC in benzene. 2,2'-Azobis(2,4-dimethylvaleronitrile) (AMVN) was used to generate the alkylperoxyl radical. The reaction products were identified as 12-formyl-11-nor-beta, beta-carotene(1), 15'-formyl-15-nor-beta, beta-carotene(2), 19-oxomethyl-10-nor-beta, beta-carotene(3), 5,6-epoxy-5,6-dihydro-beta, beta-carotene(4), 13,15'-epoxyvinyleno-13,15'-dihydro-beta, beta-carotene(5), 15'13-epoxyvinyleno-13,15'-dihydro-beta, beta-carotene(6), and 11,15'-cyclo-12,15-epoxy-11,12,15,15'-tetrahydro-beta, beta-carotene(7). The reaction products of beta-carotene during the AMVN-induced peroxidation of methyl linoleate in bulk phase were also studied. In addition to compounds 1-7, a large amount of adducts of beta-carotene with methyl linoleate were formed during the peroxidation of methyl linoleate. The results indicate that beta-carotene can scavenge lipid-peroxyl radicals to form compounds 1-7 and adducts of beta-carotene with lipids.

类胡萝卜素如β-胡萝卜素被认为是脂质过氧化的有效抑制剂。为了了解β-胡萝卜素的抗氧化活性机制，重要的是分析其作为抗氧化剂作用期间可能产生的氧化产物。本研究对β-胡萝卜素与过氧自由基的反应产物进行了分离和表征。β-胡萝卜素与烷基过氧自由基在37 ℃苯中反应。使用2，2 '-偶氮二（2，4-二甲基戊腈）（AMVN）来产生烷基过氧自由基。反应产物经鉴定为12-甲酰基-11-去甲-β，β-胡萝卜素（1），15 ′-甲酰基-15-去甲-β，β-胡萝卜素（2），19-氧代甲基-10-去甲-β，β-胡萝卜素（3），5，6-环氧-5，6-二氢-β，β-胡萝卜素（4），13，15 ′-环氧乙烯基亚基-13，15 ′-二氢-β，β-胡萝卜素（5），15 ′ 13-环氧乙烯基亚基-13，15 ′-二氢-β，β-胡萝卜素（5），15 ′-二氢-β，β-胡萝卜素（6）和11，15 ′-环-12，15-环氧-11，12，15，15 ′-四氢-β，β-胡萝卜素（7）。研究了AMVN诱导亚油酸甲酯体相过氧化过程中β-胡萝卜素的反应产物。在亚油酸甲酯过氧化过程中，除化合物1-7外，还生成了大量β-胡萝卜素与亚油酸甲酯的加合物。结果表明，β-胡萝卜素能清除脂质过氧自由基，生成化合物1-7和β-胡萝卜素与脂质的加合物。