Effect of vitamin E on the secondary oxidation process of unsaturated lipids

维生素E对不饱和脂质二次氧化过程的影响

• 批准号: 23580162
• 负责人: YAMAUCHI Ryo
• 金额: $ 3.49万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580162/
• 关键词: ビタミンＥ; α-トコフェロール; 脂質過酸化; 脂質ヒドロペルオキシド; フリーラジカル; リポソーム; 抗酸化; トコフェロール; 抗酸化剤; ミセル溶液; ビタミンE

The secondary process of lipid peroxidation forms some toxic aldehydes. Since this process proceeds via free radical intermediates, vitamin E (alpha-tocopherol, TH) would suppress the formation of such aldehydes. We have studied the effect of TH on the hemin-catalyzed decomposition of 1-palmitoyl-2-linoleoylphosphatidylcholine hydroperoxide (PLPC-OOH) in micelles and liposomes. The hemin-catalyzed decomposition of PLPC-OOH produced hexanal as the secondary oxidation product. Although TH did not suppress the decomposition of PLPC-OOH, it suppressed the formation of hexanal. The main products of TH in micelles were TH quinone and dimer, in addition to adducts of PLPC-OOH derived free radicals (T-epoxyPLPC and TOO-epoxyPLPC). In the liposomes, the main products were TOO-epoxyPLPC and dimer, and TH quinone was produced when the liposomes were destroyed. The results indicate that TH can trap PLPC-OOH derived free radicals and suppress the formation of secondary aldehydic products.

脂质过氧化的次级过程形成一些有毒的醛。由于这一过程通过自由基中间体进行，维生素E（α-生育酚，TH）将抑制这种醛的形成。本文研究了在胶束和脂质体中TH对氯化血红素催化1-棕榈酰-2-亚油酰磷脂酰胆碱氢过氧化物（PLPC-OOH）分解的影响。氯化血红素催化PLPC-OOH的分解产生作为二次氧化产物的己醛。虽然TH没有抑制PLPC-OOH的分解，但它抑制了己醛的形成。TH在胶束中的主要产物是TH醌和二聚体，以及PLPC-OOH衍生的自由基加合物（T-epoxyPLPC和TOO-epoxyPLPC）。脂质体中主要产物为TOO-环氧PLPC和二聚体，脂质体破坏后产生TH醌。结果表明，TH能捕获PLPC-OOH衍生的自由基，抑制次生代谢产物的形成。

项目成果

ヘミン触媒によるホスファチジルコリンヒドロペルオキシドの分解に対するα-トコフェロールの作用

α-生育酚对血红素催化氢过氧化磷脂酰胆碱分解的影响

• 发表时间: 2013
• 作者: 渡辺しおり;岩本悟志;山内亮
• 通讯作者: 山内亮